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Literature summary for 3.1.1.11 extracted from
- Influence of calcium on pectin methylesterase behaviour in the presence of medium methylated pectins (2011), Carbohydr. Polym., 86, 1657-1664.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | specific activity is higher in the presence than in the absence of 5 mM Ca2+ | Citrus sinensis |  |
| Ca2+ | specific activity is higher in the presence than in the absence of 5 mM Ca2+ | Aspergillus aculeatus | |
| Ca2+ | Ca2+ slightly stimulates activity at 5 mM | Citrus sinensis | |
| Ca2+ | Ca2+ strongly stimulates activity at 5 mM | Aspergillus aculeatus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Citrus sinensis | - |
methanol + pectate | - |
? | |
| pectin + H2O | Aspergillus aculeatus | - |
methanol + pectate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | Q12535 | - |
- |
| Citrus sinensis | - |
- |
- |
| Citrus sinensis | - |
cultivar Valencia | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.91 | - |
in the absence of Ca2+, pH 6.0, 30°C | Citrus sinensis |
| 2.26 | - |
in the absence of Ca2+, pH 6.0, 30°C | Aspergillus aculeatus |
| 2.54 | - |
in the presence of 5 mM Ca2+, pH 6.0, 30°C | Citrus sinensis |
| 7.59 | - |
in the presence of 5 mM Ca2+, pH 6.0, 30°C | Aspergillus aculeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Citrus sinensis | methanol + pectate | - |
? | |
| pectin + H2O | - |
Aspergillus aculeatus | methanol + pectate | - |
? | |
| pectin + H2O | a medium methylated pectin of 46% degree of methylation is used | Citrus sinensis | methanol + pectate | - |
? | |
| pectin + H2O | a medium methylated pectin of 46% degree of methylation is used | Aspergillus aculeatus | methanol + pectate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectin methylesterase | - |
Citrus sinensis |
| pectin methylesterase | - |
Aspergillus aculeatus |
| PME | - |
Citrus sinensis |
| PME | - |
Aspergillus aculeatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aspergillus aculeatus |
| 9 | - |
- |
Citrus sinensis |
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