Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Aspergillus pseudoglaucus | |
HgCl2 | 1.0 mM of HgCl2 results in approximately 50% loss of enzyme activity, while concentration of 8 mM produces complete loss of enzyme activity | Aspergillus pseudoglaucus | |
iodoacetic acid | - |
Aspergillus pseudoglaucus | |
PbCl2 | - |
Aspergillus pseudoglaucus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the highest PME activity occurrs at 20 mM of Ca2+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus | |
K+ | the activity of the enzyme increases with increase in the concentration of K+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus | |
Mg2+ | highest activity at 15 mM Mg2+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus | |
Na+ | the activity of the enzyme increases with increase in the concentration of Na+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus | |
Zn2+ | highest activity at 15 mM Zn2+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
141300 | - |
gel filtration | Aspergillus pseudoglaucus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus pseudoglaucus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sephadex G-100 gel filtration, and Sephadex C-50 gel filtration | Aspergillus pseudoglaucus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.82 | - |
crude extract | Aspergillus pseudoglaucus |
6.5 | - |
after 7.93fold purification | Aspergillus pseudoglaucus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Aspergillus pseudoglaucus | methanol + pectate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Aspergillus pseudoglaucus |
PME | - |
Aspergillus pseudoglaucus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Aspergillus pseudoglaucus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the optimum temperature for the activity of pectimethylesterase is 30°C, however, within 10 min of heating approximately 50% loss of enzyme activity is lost, additional 10 min of heating results in retention of only 9% enzyme activity, and further heating results in complete loss of enzyme activity | Aspergillus pseudoglaucus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Aspergillus pseudoglaucus |