Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Dickeya chrysanthemi |
General Stability | Organism |
---|---|
significant loss of activity after freezing and thawing | Dickeya chrysanthemi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
citrus pectin | pH 6 and pH 7.6 | Dickeya chrysanthemi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | - |
- |
- |
Dickeya chrysanthemi 3937 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity, one-step purification | Dickeya chrysanthemi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Dickeya chrysanthemi |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, stable for 1 week | Dickeya chrysanthemi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin + H2O | highest activity with pectin with an esterification degree of 50% | Dickeya chrysanthemi | methanol + citrus pectate | - |
? | |
citrus pectin + H2O | highest activity with pectin with an esterification degree of 50% | Dickeya chrysanthemi 3937 | methanol + citrus pectate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Dickeya chrysanthemi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
no loss of activity after 20 min | Dickeya chrysanthemi |
55 | - |
50% loss of activity after 5 min | Dickeya chrysanthemi |
60 | - |
complete loss of activity after 2 min | Dickeya chrysanthemi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Dickeya chrysanthemi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | about 55% activity at pH 5-6 | Dickeya chrysanthemi |