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Literature summary for 3.1.1.109 extracted from

  • Lin, H.; Fischbach, M.; Liu, D.; Walsh, C.
    In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes (2005), J. Am. Chem. Soc., 127, 11075-11084 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.109

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00008
-
 iron(III)-[monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
0.00012
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
0.00012
-
 iron(III)-[diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
0.00043
-
 iron(III)-[triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
0.04
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
0.06
-
 [monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
0.12
-
 [diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
0.16
-
 [triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A0H2V660
-
-
Escherichia coli CFT073 A0A0H2V660
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
enterobactin + H2O
-
 Escherichia coli ?
-
 ?
enterobactin + H2O
-
 Escherichia coli CFT073 ?
-
 ?
iron(III)-enterobactin + H2O
-
 Escherichia coli ?
-
 ?
iron(III)-enterobactin + H2O
-
 Escherichia coli CFT073 ?
-
 ?
iron(III)-[diglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli iron(III)-[salmochelin S2] complex
-
 ?
iron(III)-[diglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli CFT073 iron(III)-[salmochelin S2] complex
-
 ?
iron(III)-[monoglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli iron(III)-[monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex
-
 ?
iron(III)-[monoglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli CFT073 iron(III)-[monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex
-
 ?
iron(III)-[triglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli ?
-
 ?
additional information the enzyme shows a kcat/Km preference for the Fe3+-bound form of over the apo form of the substrate Escherichia coli ?
-
 ?
additional information the enzyme shows a kcat/Km preference for the Fe3+-bound form of over the apo form of the substrate Escherichia coli CFT073 ?
-
 ?
[diglucosyl-enterobactin] complex + H2O
-
 Escherichia coli [salmochelin S2] complex
-
 ?
[monoglucosyl-enterobactin] complex + H2O
-
 Escherichia coli [monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex
-
 ?
[triglucosyl-enterobactin] complex + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
Fe-MGE/Fe-DGE esterase
-
 Escherichia coli
ferric MGE/DGE esterase
-
 Escherichia coli
IroD
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
 iron(III)-[diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
0.77
-
 iron(III)-[monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
1.23
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
1.4
-
 iron(III)-[triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
17.67
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
62
-
 [monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
74.33
-
 [triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
108.33
-
 [diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
433
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
467
-
 [triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
900
-
 [diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
1033
-
 [monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3250
-
 iron(III)-[triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
4450
-
 iron(III)-[diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
9583
-
 iron(III)-[monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
10283
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli