Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.108 extracted from

  • Lin, H.; Fischbach, M.; Liu, D.; Walsh, C.
    In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes (2005), J. Am. Chem. Soc., 127, 11075-11084 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.108

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00029
-
 iron(III)-monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.001
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
0.1
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.13
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
0.13
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli CFT073
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Mono Q column chromatography, and Superdex S200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diglucosyl-enterobactin + H2O poor substrate Escherichia coli diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
diglucosyl-enterobactin + H2O poor substrate Escherichia coli CFT073 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
enterobactin + H2O
-
 Escherichia coli N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
enterobactin + H2O
-
 Escherichia coli CFT073 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
iron(III)-enterobactin + H2O highest catalytic efficiency Escherichia coli iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
iron(III)-enterobactin + H2O highest catalytic efficiency Escherichia coli CFT073 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
iron(III)-monoglucosyl-enterobactin + H2O
-
 Escherichia coli ?
-
 ?
monoglucosyl-enterobactin + H2O
-
 Escherichia coli monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
monoglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
additional information no activity with triglucosyl-enterobactin, iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex and iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex Escherichia coli ?
-
 ?
additional information no activity with triglucosyl-enterobactin, iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex and iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex Escherichia coli CFT073 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Escherichia coli
? x * 43000, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
ferric Ent esterase
-
 Escherichia coli
Fes
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
 iron(III)-monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.15
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
1.3
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
8.67
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
14.17
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
66.7
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
80
-
 iron(III)-monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
141.7
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
1500
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli