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Literature summary for 3.1.1.107 extracted from

  • Lin, H.; Fischbach, M.; Liu, D.; Walsh, C.
    In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes (2005), J. Am. Chem. Soc., 127, 11075-11084 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.107

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 ferric enterobactin at pH 7.5 and 23°C Escherichia coli
0.0046
-
 ferric diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.0048
-
 ferric monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.016
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
0.016
-
 ferric triglucosyl-enterobactin Km above 0.016 mM, at pH 7.5 and 23°C Escherichia coli
0.029
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.039
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.155
-
 triglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diglucosyl-enterobactin + H2O Escherichia coli
-
 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
diglucosyl-enterobactin + H2O Escherichia coli CFT073
-
 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
enterobactin + H2O Escherichia coli
-
 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
enterobactin + H2O Escherichia coli CFT073
-
 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
monoglucosyl-enterobactin + H2O Escherichia coli
-
 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
monoglucosyl-enterobactin + H2O Escherichia coli CFT073
-
 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
triglucosyl-enterobactin + H2O Escherichia coli
-
 triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A0H2V871
-
-
Escherichia coli CFT073 A0A0H2V871
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diglucosyl-enterobactin + H2O
-
 Escherichia coli diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
diglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
enterobactin + H2O
-
 Escherichia coli N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
enterobactin + H2O the enzyme prefers apo siderophores as substrates, and tends to hydrolyze the trilactone just once to produce linearized trimers Escherichia coli N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
enterobactin + H2O
-
 Escherichia coli CFT073 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
enterobactin + H2O the enzyme prefers apo siderophores as substrates, and tends to hydrolyze the trilactone just once to produce linearized trimers Escherichia coli CFT073 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
ferric diglucosyl-enterobactin + H2O
-
 Escherichia coli ferric diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
ferric enterobactin + H2O
-
 Escherichia coli ferric N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
ferric monoglucosyl-enterobactin + H2O
-
 Escherichia coli ferric monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
ferric triglucosyl-enterobactin + H2O
-
 Escherichia coli triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
monoglucosyl-enterobactin + H2O
-
 Escherichia coli monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
monoglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
additional information the enzyme hydrolyzes the Fe3+-bound siderophores very inefficiently. The enzyme hydrolyzes apo forms of siderophores more efficiently than Fe3+-bound ones Escherichia coli ?
-
 ?
additional information the enzyme hydrolyzes the Fe3+-bound siderophores very inefficiently. The enzyme hydrolyzes apo forms of siderophores more efficiently than Fe3+-bound ones Escherichia coli CFT073 ?
-
 ?
triglucosyl-enterobactin + H2O
-
 Escherichia coli triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
iroE
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 ferric diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.05
-
 ferric enterobactin at pH 7.5 and 23°C Escherichia coli
0.05
-
 ferric monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
0.13
-
 ferric triglucosyl-enterobactin turnover number above 0.13 1/sec, at pH 7.5 and 23°C Escherichia coli
5.33
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
6.25
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
7.17
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
7.5
-
 triglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.3
-
 ferric diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
8.3
-
 ferric triglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
11.7
-
 ferric monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
15
-
 ferric enterobactin at pH 7.5 and 23°C Escherichia coli
50
-
 triglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
133
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
233
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
383
-
 enterobactin at pH 7.5 and 23°C Escherichia coli