BRENDA - Enzyme Database
show all sequences of 3.1.1.106

Proximal ADP-ribose hydrolysis in Trypanosomatids is catalyzed by a macrodomain

Haikarainen, T.; Lehtioe, L.; Sci. Rep. 6, 24213 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Trypanosoma brucei gambiense
expressed in Escherichia coli BL21(DE3) cells
Trypanosoma cruzi
Crystallization (Commentary)
Crystallization
Organism
apoenzyme and in complex with ADP-D-ribose or ADP, sitting drop vapor diffusion method, using 20-25% (w/v) PEG 3350
Trypanosoma brucei gambiense
apoenzyme, sitting drop vapor diffusion method, using 0.1 M Bis-Tris propane pH 8.0, 0.96 M trisodium citrate
Trypanosoma cruzi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma cruzi
-
ADP-D-ribose + acetate
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma brucei gambiense
-
ADP-D-ribose + acetate
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma cruzi CL Brener
-
ADP-D-ribose + acetate
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma cruzi
-
ADP-D-ribose + acetate
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma brucei gambiense
-
ADP-D-ribose + acetate
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma cruzi CL Brener
-
ADP-D-ribose + acetate
-
-
?
[protein]-ADP-D-ribose + H2O
Trypanosoma cruzi
-
[protein] + ADP-D-ribose
-
-
?
[protein]-ADP-D-ribose + H2O
Trypanosoma brucei gambiense
-
[protein] + ADP-D-ribose
-
-
?
[protein]-ADP-D-ribose + H2O
Trypanosoma cruzi CL Brener
-
[protein] + ADP-D-ribose
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trypanosoma brucei gambiense
C9ZP98
-
-
Trypanosoma cruzi
Q4DQ03
-
-
Trypanosoma cruzi CL Brener
Q4DQ03
-
-
Purification (Commentary)
Commentary
Organism
HisTrap column chromatography and Superdex 75 gel filtration
Trypanosoma brucei gambiense
HisTrap column chromatography and Superdex 75 gel filtration
Trypanosoma cruzi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi
ADP-D-ribose + acetate
-
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma brucei gambiense
ADP-D-ribose + acetate
-
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi CL Brener
ADP-D-ribose + acetate
-
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi
ADP-D-ribose + acetate
-
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma brucei gambiense
ADP-D-ribose + acetate
-
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi CL Brener
ADP-D-ribose + acetate
-
-
-
?
[protein]-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi
[protein] + ADP-D-ribose
-
-
-
?
[protein]-ADP-D-ribose + H2O
-
752189
Trypanosoma brucei gambiense
[protein] + ADP-D-ribose
-
-
-
?
[protein]-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi CL Brener
[protein] + ADP-D-ribose
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Trypanosoma brucei gambiense
expressed in Escherichia coli BL21(DE3) cells
Trypanosoma cruzi
Crystallization (Commentary) (protein specific)
Crystallization
Organism
apoenzyme and in complex with ADP-D-ribose or ADP, sitting drop vapor diffusion method, using 20-25% (w/v) PEG 3350
Trypanosoma brucei gambiense
apoenzyme, sitting drop vapor diffusion method, using 0.1 M Bis-Tris propane pH 8.0, 0.96 M trisodium citrate
Trypanosoma cruzi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma cruzi
-
ADP-D-ribose + acetate
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma brucei gambiense
-
ADP-D-ribose + acetate
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma cruzi CL Brener
-
ADP-D-ribose + acetate
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma cruzi
-
ADP-D-ribose + acetate
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma brucei gambiense
-
ADP-D-ribose + acetate
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
Trypanosoma cruzi CL Brener
-
ADP-D-ribose + acetate
-
-
?
[protein]-ADP-D-ribose + H2O
Trypanosoma cruzi
-
[protein] + ADP-D-ribose
-
-
?
[protein]-ADP-D-ribose + H2O
Trypanosoma brucei gambiense
-
[protein] + ADP-D-ribose
-
-
?
[protein]-ADP-D-ribose + H2O
Trypanosoma cruzi CL Brener
-
[protein] + ADP-D-ribose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
HisTrap column chromatography and Superdex 75 gel filtration
Trypanosoma brucei gambiense
HisTrap column chromatography and Superdex 75 gel filtration
Trypanosoma cruzi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi
ADP-D-ribose + acetate
-
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma brucei gambiense
ADP-D-ribose + acetate
-
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi CL Brener
ADP-D-ribose + acetate
-
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi
ADP-D-ribose + acetate
-
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma brucei gambiense
ADP-D-ribose + acetate
-
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi CL Brener
ADP-D-ribose + acetate
-
-
-
?
[protein]-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi
[protein] + ADP-D-ribose
-
-
-
?
[protein]-ADP-D-ribose + H2O
-
752189
Trypanosoma brucei gambiense
[protein] + ADP-D-ribose
-
-
-
?
[protein]-ADP-D-ribose + H2O
-
752189
Trypanosoma cruzi CL Brener
[protein] + ADP-D-ribose
-
-
-
?
Other publictions for EC 3.1.1.106
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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4
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5
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8
2
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3
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1
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3
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4
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1
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4
5
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3
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1
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3
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752189
Haikarainen
Proximal ADP-ribose hydrolysi ...
Trypanosoma brucei gambiense, Trypanosoma cruzi, Trypanosoma cruzi CL Brener
Sci. Rep.
6
24213
2016
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2
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9
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8
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9
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9
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9
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751419
Zhang
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Escherichia coli
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1
4
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3
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3
749471
Hirsch
Transition-state analysis of ...
Homo sapiens
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2255-2262
2014
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1
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2
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2
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2
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2
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2
2
751688
Rosenthal
Macrodomain-containing protei ...
Homo sapiens
Nat. Struct. Mol. Biol.
20
502-507
2013
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1
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1
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6
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5
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1
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13
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3
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1
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6
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3
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13
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1
3
-
-
-
751029
Chen
Identification of macrodomain ...
Escherichia coli, Homo sapiens, Staphylococcus aureus
J. Biol. Chem.
286
13261-13271
2011
-
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1
1
15
-
3
4
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8
-
8
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1
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1
-
-
12
-
-
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18
-
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1
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1
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1
15
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4
1
4
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8
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1
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2
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12
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18
-
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4
4
751030
Kasamatsu
Hydrolysis of O-acetyl-ADP-ri ...
Homo sapiens
J. Biol. Chem.
286
21110-21117
2011
-
-
-
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4
1
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1
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1
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1
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6
1
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1
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3
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3
4
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1
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1
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1
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6
1
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1
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752026
Ono
The 39-kDa poly(ADP-ribose) g ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
16687-16691
2006
1
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3
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2
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1
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2
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1
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2
1
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1
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3
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2
1
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1
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2
1
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751012
Rafty
-
Analysis of O-acetyl-ADP-ribo ...
Homo sapiens, Saccharomyces cerevisiae
J. Biol. Chem.
277
47114-47122
2002
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2
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