BRENDA - Reference to 3.1.1.106; Id = 751419

expressed in Escherichia coli Rosetta2 (DE3) cells

Escherichia coli

sitting drop vapor diffusion method. Wild type enzyme in complex with ADP-D-ribose using 0.2 M potassium sodium tartrate tetrahydrate, 20% w/v polyethylene glycol 3350, double mutant N25A/D35A complexed with 2''-O-acetyl-ADP-D-ribose using 2 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5, and mutant Y126A in complex with ADP-D-ribose using 2 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5

Escherichia coli

D35A

the mutant shows strongly reduced activity compared to the wild type enzyme

Escherichia coli

N25A

the mutant shows strongly reduced activity compared to the wild type enzyme

Escherichia coli

N25A/D35A

inactive

Escherichia coli

Y126A

inactive

Escherichia coli

0.4306

-

2''-O-acetyl-ADP-D-ribose

wild type enzyme, at pH 7.5 and 25°C

Escherichia coli

1.358

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme N25A, at pH 7.5 and 25°C

Escherichia coli

2.74

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme D35A, at pH 7.5 and 25°C

Escherichia coli

2''-O-acetyl-ADP-D-ribose + H2O

Escherichia coli

specific substrate

ADP-D-ribose + acetate

-

?

Escherichia coli

P0A8D6

-

Ni-NTA resin column chromatography and Superdex S200 gel filtration

Escherichia coli

2''-O-acetyl-ADP-D-ribose + H2O

-

751419

Escherichia coli

ADP-D-ribose + acetate

-

?

2''-O-acetyl-ADP-D-ribose + H2O

specific substrate

751419

Escherichia coli

ADP-D-ribose + acetate

-

?

0.2

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme D35A, at pH 7.5 and 25°C

Escherichia coli

0.814

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme N25A, at pH 7.5 and 25°C

Escherichia coli

1.31

-

2''-O-acetyl-ADP-D-ribose

wild type enzyme, at pH 7.5 and 25°C

Escherichia coli

expressed in Escherichia coli Rosetta2 (DE3) cells

Escherichia coli

sitting drop vapor diffusion method. Wild type enzyme in complex with ADP-D-ribose using 0.2 M potassium sodium tartrate tetrahydrate, 20% w/v polyethylene glycol 3350, double mutant N25A/D35A complexed with 2''-O-acetyl-ADP-D-ribose using 2 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5, and mutant Y126A in complex with ADP-D-ribose using 2 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5

Escherichia coli

D35A

the mutant shows strongly reduced activity compared to the wild type enzyme

Escherichia coli

N25A

the mutant shows strongly reduced activity compared to the wild type enzyme

Escherichia coli

N25A/D35A

inactive

Escherichia coli

Y126A

inactive

Escherichia coli

0.4306

-

2''-O-acetyl-ADP-D-ribose

wild type enzyme, at pH 7.5 and 25°C

Escherichia coli

1.358

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme N25A, at pH 7.5 and 25°C

Escherichia coli

2.74

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme D35A, at pH 7.5 and 25°C

Escherichia coli

2''-O-acetyl-ADP-D-ribose + H2O

Escherichia coli

specific substrate

ADP-D-ribose + acetate

-

?

Ni-NTA resin column chromatography and Superdex S200 gel filtration

Escherichia coli

2''-O-acetyl-ADP-D-ribose + H2O

-

751419

Escherichia coli

ADP-D-ribose + acetate

-

?

2''-O-acetyl-ADP-D-ribose + H2O

specific substrate

751419

Escherichia coli

ADP-D-ribose + acetate

-

?

0.2

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme D35A, at pH 7.5 and 25°C

Escherichia coli

0.814

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme N25A, at pH 7.5 and 25°C

Escherichia coli

1.31

-

2''-O-acetyl-ADP-D-ribose

wild type enzyme, at pH 7.5 and 25°C

Escherichia coli

0.073

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme D35A, at pH 7.5 and 25°C

Escherichia coli

0.599

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme N25A, at pH 7.5 and 25°C

Escherichia coli

3.042

-

2''-O-acetyl-ADP-D-ribose

wild type enzyme, at pH 7.5 and 25°C

Escherichia coli

0.073

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme D35A, at pH 7.5 and 25°C

Escherichia coli

0.599

-

2''-O-acetyl-ADP-D-ribose

mutant enzyme N25A, at pH 7.5 and 25°C

Escherichia coli

3.042

-

2''-O-acetyl-ADP-D-ribose

wild type enzyme, at pH 7.5 and 25°C

Escherichia coli

750645

Agnew

MacroD1 is a promiscuous ADP- ...

Homo sapiens, Mus musculus

Front. Microbiol.

9

20

2018

-

2

-

4

-

4

-

5

-

8

2

-

2

-

4

-

5

-

8

2

-

752262

Haikarainen

Development of an inhibitor s ...

Homo sapiens

SLAS Discov.

23

255-263

2018

-

1

-

5

-

3

-

2

-

1

-

3

-

4

-

1

-

4

5

-

3

-

1

-

3

-

752189

Haikarainen

Proximal ADP-ribose hydrolysi ...

Trypanosoma brucei gambiense, Trypanosoma cruzi, Trypanosoma cruzi CL Brener

Sci. Rep.

6

24213

2016

-

2

-

9

-

8

-

2

-

9

-

2

-

2

-

9

-

2

-

9

-

751419

Zhang

Structural insights into the ...

Escherichia coli

J. Struct. Biol.

192

478-486

2015

-

1

4

-

3

-

1

-

1

-

1

-

2

-

3

-

1

-

1

4

-

3

-

1

-

1

-

2

-

3

-

3

749471

Hirsch

Transition-state analysis of ...

Homo sapiens

ACS Chem. Biol.

9

2255-2262

2014

-

1

-

2

-

2

-

2

-

2

-

2

-

1

-

2

-

2

-

2

-

2

-

2

751688

Rosenthal

Macrodomain-containing protei ...

Homo sapiens

Nat. Struct. Mol. Biol.

20

502-507

2013

-

1

-

1

-

6

-

5

-

1

-

13

-

3

-

1

-

6

-

3

-

13

-

1

3

-

751029

Chen

Identification of macrodomain ...

Escherichia coli, Homo sapiens, Staphylococcus aureus

J. Biol. Chem.

286

13261-13271

2011

-

1

15

-

3

4

-

8

-

8

-

1

-

1

-

12

-

18

-

1

-

1

-

1

15

-

4

1

4

-

8

-

1

-

2

-

12

-

18

-

4

751030

Kasamatsu

Hydrolysis of O-acetyl-ADP-ri ...

Homo sapiens

J. Biol. Chem.

286

21110-21117

2011

-

4

1

-

1

-

1

-

1

-

6

1

-

1

-

3

-

3

4

-

1

-

1

-

1

-

6

1

-

1

-

752026

Ono

The 39-kDa poly(ADP-ribose) g ...

Homo sapiens

Proc. Natl. Acad. Sci. USA

103

16687-16691

2006

1

-

3

-

2

-

1

-

2

-

1

-

2

1

-

1

-

1

-

3

-

2

1

-

1

-

2

-

2

1

-

751012

Rafty

-

Analysis of O-acetyl-ADP-ribo ...

Homo sapiens, Saccharomyces cerevisiae

J. Biol. Chem.

277

47114-47122

2002

-

2

-

4

-

2

-

3

-

4

-

2

-

4

-

3

-

4

-

Structural insights into the mechanism of Escherichia coli YmdB A 2-O-acetyl-ADP-ribose deacetylase

Data extracted from this reference: