BRENDA - Enzyme Database
show all sequences of 3.1.1.106

Hydrolysis of O-acetyl-ADP-ribose isomers by ADP-ribosylhydrolase 3

Kasamatsu, A.; Nakao, M.; Smith, B.C.; Comstock, L.R.; Ono, T.; Kato, J.; Denu, J.M.; Moss, J.; J. Biol. Chem. 286, 21110-21117 (2011)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2''-N-acetyl-ADP-D-ribose
poor inhibitor
Homo sapiens
3''-N-acetyl-ADP-D-ribose
poor inhibitor
Homo sapiens
ADP-D-ribose
potent inhibitor
Homo sapiens
additional information
not inhibited by D-ribose 5-phosphate, AMP, ADP, or beta-NAD+
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0043
-
3''-O-acetyl-ADP-D-ribose
at pH 7.0 and 30°C
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
dependent on
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1''-O-acetyl-ADP-D-ribose + H2O
Homo sapiens
-
ADP-D-ribose + acetate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1''-O-acetyl-ADP-D-ribose + H2O
-
751030
Homo sapiens
ADP-D-ribose + acetate
-
-
-
?
1''-O-acetyl-ADP-D-ribose + H2O
predominant substrate at pH 9.0
751030
Homo sapiens
ADP-D-ribose + acetate
-
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
predominant substrate at pH 7.0
751030
Homo sapiens
ADP-D-ribose + acetate
-
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
predominant substrate at pH 5.0 and 7.0
751030
Homo sapiens
ADP-D-ribose + acetate
-
-
-
?
additional information
neither 2''- nor 3''-N-acetyl-ADP-D-ribose is a substrate for the enzyme
751030
Homo sapiens
?
-
-
-
-
poly(ADP-D-ribose) + H2O
the linkage hydrolyzed by the enzyme in poly(ADP-D-ribose) is stereospecific at the C-1' position with the alpha-anomer present in the polymer
751030
Homo sapiens
ADP-D-ribose
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 39000, calculated from amino acid sequence
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Homo sapiens
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.012
-
at pH 7.0 and 30°C
Homo sapiens
ADP-D-ribose
0.71
-
at pH 7.0 and 30°C
Homo sapiens
2''-N-acetyl-ADP-D-ribose
2.3
-
at pH 7.0 and 30°C
Homo sapiens
3''-N-acetyl-ADP-D-ribose
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.012
-
at pH 7.0 and 30°C
Homo sapiens
ADP-D-ribose
0.71
-
at pH 7.0 and 30°C
Homo sapiens
2''-N-acetyl-ADP-D-ribose
2.3
-
at pH 7.0 and 30°C
Homo sapiens
3''-N-acetyl-ADP-D-ribose
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2''-N-acetyl-ADP-D-ribose
poor inhibitor
Homo sapiens
3''-N-acetyl-ADP-D-ribose
poor inhibitor
Homo sapiens
ADP-D-ribose
potent inhibitor
Homo sapiens
additional information
not inhibited by D-ribose 5-phosphate, AMP, ADP, or beta-NAD+
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0043
-
3''-O-acetyl-ADP-D-ribose
at pH 7.0 and 30°C
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
dependent on
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1''-O-acetyl-ADP-D-ribose + H2O
Homo sapiens
-
ADP-D-ribose + acetate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1''-O-acetyl-ADP-D-ribose + H2O
-
751030
Homo sapiens
ADP-D-ribose + acetate
-
-
-
?
1''-O-acetyl-ADP-D-ribose + H2O
predominant substrate at pH 9.0
751030
Homo sapiens
ADP-D-ribose + acetate
-
-
-
?
2''-O-acetyl-ADP-D-ribose + H2O
predominant substrate at pH 7.0
751030
Homo sapiens
ADP-D-ribose + acetate
-
-
-
?
3''-O-acetyl-ADP-D-ribose + H2O
predominant substrate at pH 5.0 and 7.0
751030
Homo sapiens
ADP-D-ribose + acetate
-
-
-
?
additional information
neither 2''- nor 3''-N-acetyl-ADP-D-ribose is a substrate for the enzyme
751030
Homo sapiens
?
-
-
-
-
poly(ADP-D-ribose) + H2O
the linkage hydrolyzed by the enzyme in poly(ADP-D-ribose) is stereospecific at the C-1' position with the alpha-anomer present in the polymer
751030
Homo sapiens
ADP-D-ribose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 39000, calculated from amino acid sequence
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Homo sapiens
Other publictions for EC 3.1.1.106
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750645
Agnew
MacroD1 is a promiscuous ADP- ...
Homo sapiens, Mus musculus
Front. Microbiol.
9
20
2018
-
-
-
-
-
-
-
-
2
-
-
4
-
4
-
-
-
-
-
5
-
-
8
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
5
-
-
8
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
752262
Haikarainen
Development of an inhibitor s ...
Homo sapiens
SLAS Discov.
23
255-263
2018
-
-
1
-
-
-
5
-
-
-
-
3
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
4
5
-
-
-
-
-
3
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
752189
Haikarainen
Proximal ADP-ribose hydrolysi ...
Trypanosoma brucei gambiense, Trypanosoma cruzi, Trypanosoma cruzi CL Brener
Sci. Rep.
6
24213
2016
-
-
2
2
-
-
-
-
-
-
-
9
-
8
-
-
2
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
9
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751419
Zhang
Structural insights into the ...
Escherichia coli
J. Struct. Biol.
192
478-486
2015
-
-
1
1
4
-
-
3
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
-
3
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
749471
Hirsch
Transition-state analysis of ...
Homo sapiens
ACS Chem. Biol.
9
2255-2262
2014
-
-
1
-
-
-
-
2
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
751688
Rosenthal
Macrodomain-containing protei ...
Homo sapiens
Nat. Struct. Mol. Biol.
20
502-507
2013
-
-
1
-
1
-
-
-
-
-
-
6
-
5
-
-
1
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
3
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
751029
Chen
Identification of macrodomain ...
Escherichia coli, Homo sapiens, Staphylococcus aureus
J. Biol. Chem.
286
13261-13271
2011
-
-
1
1
15
-
3
4
-
-
-
8
-
8
-
-
1
-
-
1
-
-
12
-
-
-
-
18
-
-
-
-
1
-
-
-
-
1
-
1
15
-
-
4
1
4
-
-
-
8
-
-
-
1
-
2
-
-
12
-
-
-
-
18
-
-
-
-
-
-
-
-
4
4
751030
Kasamatsu
Hydrolysis of O-acetyl-ADP-ri ...
Homo sapiens
J. Biol. Chem.
286
21110-21117
2011
-
-
-
-
-
-
4
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
3
4
-
1
-
1
-
1
-
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
752026
Ono
The 39-kDa poly(ADP-ribose) g ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
16687-16691
2006
1
-
-
-
3
-
2
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
2
1
-
-
1
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751012
Rafty
-
Analysis of O-acetyl-ADP-ribo ...
Homo sapiens, Saccharomyces cerevisiae
J. Biol. Chem.
277
47114-47122
2002
-
-
-
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
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3
-
-
4
-
-
-
-
-
-
-
-
-
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-