Transition-state analysis of 2-O-acetyl-ADP-ribose hydrolysis by human macrodomain 1

Hirsch, B.M.; Burgos, E.S.; Schramm, V.L.; ACS Chem. Biol. 9, 2255-2262 (2014)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.37

-

2''-O-acetyl-ADP-D-ribose

at pH 7.3 and 25°C

Homo sapiens

1.4

-

2''-O-acetyl-ADP-D-ribose

at pH 6.8 and 25°C

Homo sapiens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2''-O-acetyl-ADP-D-ribose + H2O

Homo sapiens

-

ADP-D-ribose + acetate

-

-

ir

3''-O-acetyl-ADP-D-ribose + H2O

Homo sapiens

-

ADP-D-ribose + acetate

-

-

ir

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2''-O-acetyl-ADP-D-ribose + H2O

-

749471

Homo sapiens

ADP-D-ribose + acetate

-

-

-

ir

3''-O-acetyl-ADP-D-ribose + H2O

-

749471

Homo sapiens

ADP-D-ribose + acetate

-

-

-

ir

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.2

-

2''-O-acetyl-ADP-D-ribose

at pH 7.3 and 25°C

Homo sapiens

0.72

-

2''-O-acetyl-ADP-D-ribose

at pH 6.8 and 25°C

Homo sapiens

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.37

-

2''-O-acetyl-ADP-D-ribose

at pH 7.3 and 25°C

Homo sapiens

1.4

-

2''-O-acetyl-ADP-D-ribose

at pH 6.8 and 25°C

Homo sapiens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2''-O-acetyl-ADP-D-ribose + H2O

Homo sapiens

-

ADP-D-ribose + acetate

-

-

ir

3''-O-acetyl-ADP-D-ribose + H2O

Homo sapiens

-

ADP-D-ribose + acetate

-

-

ir

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2''-O-acetyl-ADP-D-ribose + H2O

-

749471

Homo sapiens

ADP-D-ribose + acetate

-

-

-

ir

3''-O-acetyl-ADP-D-ribose + H2O

-

749471

Homo sapiens

ADP-D-ribose + acetate

-

-

-

ir

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.2

-

2''-O-acetyl-ADP-D-ribose

at pH 7.3 and 25°C

Homo sapiens

0.72

-

2''-O-acetyl-ADP-D-ribose

at pH 6.8 and 25°C

Homo sapiens

kcat/KM Value [1/mMs^-1]

kcat/KM Value Maximum [1/mMs^-1]

Substrate

Commentary

Organism

Structure

0.49

-

2''-O-acetyl-ADP-D-ribose

at pH 6.8 and 25°C

Homo sapiens

0.53

-

2''-O-acetyl-ADP-D-ribose

at pH 7.3 and 25°C

Homo sapiens

KCat/KM Value [1/mMs^-1]

KCat/KM Value Maximum [1/mMs^-1]

Substrate

Commentary

Organism

Structure

0.49

-

2''-O-acetyl-ADP-D-ribose

at pH 6.8 and 25°C

Homo sapiens

0.53

-

2''-O-acetyl-ADP-D-ribose

at pH 7.3 and 25°C

Homo sapiens

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

750645

Agnew

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Transition-state analysis of ...

Homo sapiens

ACS Chem. Biol.

9

2255-2262

2014

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