BRENDA - Enzyme Database
show all sequences of 3.1.1.106

Transition-state analysis of 2-O-acetyl-ADP-ribose hydrolysis by human macrodomain 1

Hirsch, B.M.; Burgos, E.S.; Schramm, V.L.; ACS Chem. Biol. 9, 2255-2262 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.37
-
2''-O-acetyl-ADP-D-ribose
at pH 7.3 and 25°C
Homo sapiens
1.4
-
2''-O-acetyl-ADP-D-ribose
at pH 6.8 and 25°C
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2''-O-acetyl-ADP-D-ribose + H2O
Homo sapiens
-
ADP-D-ribose + acetate
-
-
ir
3''-O-acetyl-ADP-D-ribose + H2O
Homo sapiens
-
ADP-D-ribose + acetate
-
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
Q9BQ69
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2''-O-acetyl-ADP-D-ribose + H2O
-
749471
Homo sapiens
ADP-D-ribose + acetate
-
-
-
ir
3''-O-acetyl-ADP-D-ribose + H2O
-
749471
Homo sapiens
ADP-D-ribose + acetate
-
-
-
ir
Synonyms
Synonyms
Commentary
Organism
MACROD1
-
Homo sapiens
macrodomain 1
-
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
2''-O-acetyl-ADP-D-ribose
at pH 7.3 and 25°C
Homo sapiens
0.72
-
2''-O-acetyl-ADP-D-ribose
at pH 6.8 and 25°C
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.37
-
2''-O-acetyl-ADP-D-ribose
at pH 7.3 and 25°C
Homo sapiens
1.4
-
2''-O-acetyl-ADP-D-ribose
at pH 6.8 and 25°C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2''-O-acetyl-ADP-D-ribose + H2O
Homo sapiens
-
ADP-D-ribose + acetate
-
-
ir
3''-O-acetyl-ADP-D-ribose + H2O
Homo sapiens
-
ADP-D-ribose + acetate
-
-
ir
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2''-O-acetyl-ADP-D-ribose + H2O
-
749471
Homo sapiens
ADP-D-ribose + acetate
-
-
-
ir
3''-O-acetyl-ADP-D-ribose + H2O
-
749471
Homo sapiens
ADP-D-ribose + acetate
-
-
-
ir
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
2''-O-acetyl-ADP-D-ribose
at pH 7.3 and 25°C
Homo sapiens
0.72
-
2''-O-acetyl-ADP-D-ribose
at pH 6.8 and 25°C
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.49
-
2''-O-acetyl-ADP-D-ribose
at pH 6.8 and 25°C
Homo sapiens
0.53
-
2''-O-acetyl-ADP-D-ribose
at pH 7.3 and 25°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.49
-
2''-O-acetyl-ADP-D-ribose
at pH 6.8 and 25°C
Homo sapiens
0.53
-
2''-O-acetyl-ADP-D-ribose
at pH 7.3 and 25°C
Homo sapiens
Other publictions for EC 3.1.1.106
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Agnew
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-
-
-
-
-
-
-
2
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4
-
6
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5
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8
2
4
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5
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-
-
-
5
-
-
-
-
3
-
3
-
-
1
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-
-
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-
3
-
1
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4
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1
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4
5
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3
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1
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3
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752189
Haikarainen
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Trypanosoma brucei gambiense, Trypanosoma cruzi, Trypanosoma cruzi CL Brener
Sci. Rep.
6
24213
2016
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2
2
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9
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11
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2
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9
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6
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2
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2
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9
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2
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9
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751419
Zhang
Structural insights into the ...
Escherichia coli
J. Struct. Biol.
192
478-486
2015
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-
1
1
4
-
-
3
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
2
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3
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1
-
1
4
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-
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3
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1
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-
1
-
-
-
-
2
-
-
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-
3
-
-
-
-
-
-
-
-
3
3
749471
Hirsch
Transition-state analysis of ...
Homo sapiens
ACS Chem. Biol.
9
2255-2262
2014
-
-
1
-
-
-
-
2
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
2
-
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-
2
-
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1
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2
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2
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2
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2
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2
2
751688
Rosenthal
Macrodomain-containing protei ...
Homo sapiens
Nat. Struct. Mol. Biol.
20
502-507
2013
-
-
3
-
1
-
-
-
-
-
-
6
-
7
-
-
3
-
-
-
-
-
13
-
6
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-
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-
-
-
-
-
-
-
-
-
-
3
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-
1
-
-
-
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6
-
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-
3
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-
-
-
13
-
-
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-
-
-
-
-
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-
3
3
-
-
-
751029
Chen
Identification of macrodomain ...
Escherichia coli, Staphylococcus aureus, Homo sapiens
J. Biol. Chem.
286
13261-13271
2011
-
-
1
1
15
-
3
4
-
-
-
8
-
11
-
-
1
-
-
2
-
-
12
-
13
-
-
-
19
-
-
-
-
1
-
-
-
-
1
-
1
15
-
-
4
1
4
-
-
-
8
-
-
-
1
-
2
-
-
12
-
-
-
-
19
-
-
-
-
-
-
-
-
4
4
751030
Kasamatsu
Hydrolysis of O-acetyl-ADP-ri ...
Homo sapiens
J. Biol. Chem.
286
21110-21117
2011
-
-
-
-
-
-
4
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
6
1
3
-
-
-
-
1
-
-
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3
-
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-
-
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-
3
4
-
1
-
1
-
1
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-
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-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
752026
Ono
The 39-kDa poly(ADP-ribose) g ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
16687-16691
2006
1
-
-
-
3
-
2
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
2
1
3
-
-
-
-
-
-
-
-
1
-
-
1
-
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-
-
3
-
-
2
1
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1
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751012
Rafty
-
Analysis of O-acetyl-ADP-ribo ...
Saccharomyces cerevisiae, Homo sapiens
J. Biol. Chem.
277
47114-47122
2002
-
-
-
-
-
-
-
-
2
-
-
4
-
2
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3
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4
-
2
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2
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4
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3
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4
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