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Literature summary for 3.1.1.101 extracted from

  • Liu, B.; He, L.; Wang, L.; Li, T.; Li, C.; Liu, H.; Luo, Y.; Bao, R.
    Protein crystallography and site-direct mutagenesis analysis of the poly(ethylene terephthalate) hydrolase PETase from Ideonella sakaiensis (2018), ChemBioChem, 19, 1471-1475 .
    View publication on PubMed

Application

Application Comment Organism
degradation the enzyme can offer an important contribution towards a future sustainable closed loop plastic recycling industry Ideonella sakaiensis
environmental protection the enzyme can offer an important contribution towards a future sustainable closed loop plastic recycling industry Ideonella sakaiensis

Protein Variants

Protein Variants Comment Organism
A180I mutation results in more space on the binding center and higher activity than the wild-type enzyme Ideonella sakaiensis
D177A inactive mutant enzyme Ideonella sakaiensis
H208A inactive mutant enzyme Ideonella sakaiensis
M132A marked decrease in hydrolysis activity Ideonella sakaiensis
Q90A marked decrease in hydrolysis activity Ideonella sakaiensis
S131A inactive mutant enzyme Ideonella sakaiensis
S209F strongly reduced hydrolysis of the PET particles Ideonella sakaiensis
W130A mutation results in more space on the binding center and higher activity than the wild-type enzyme Ideonella sakaiensis
W130H mutation results in more space on the binding center and higher activity than the wild-type enzyme Ideonella sakaiensis
W156A marked decrease in hydrolysis activity Ideonella sakaiensis
Y58A mutation results in more space on the binding center and higher activity than the wild-type enzyme Ideonella sakaiensis

Organism

Organism UniProt Comment Textmining
Ideonella sakaiensis A0A0K8P6T7
-
-
Ideonella sakaiensis 201-F6 A0A0K8P6T7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ethylene terephthalate)n + H2O
-
Ideonella sakaiensis (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
?
(ethylene terephthalate)n + H2O
-
Ideonella sakaiensis 201-F6 (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
?

Synonyms

Synonyms Comment Organism
PETase
-
Ideonella sakaiensis