Literature summary for 3.1.1.101 extracted from

Seo, H.; Kim, S.; Son, H.F.; Sagong, H.Y.; Joo, S.; Kim, K.J.
Production of extracellular PETase from Ideonella sakaiensis using sec-dependent signal peptides in E. coli (2018), Biochem. Biophys. Res. Commun., 508, 250-255 .

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Application

Application	Comment	Organism
degradation	due to its low structural stability and solubility, it is difficult to apply standard laboratory-level Ideonella sakaiensis PETase expression and purification procedures in industry. To overcome this difficulty, the expression of IsPETase can be improved by using a secretion system. The extracellular enzyme is successfully produced using pET22b-SPMalE:IsPETase and pET22b-SPLamB:IsPETase expression systems. The secreted IsPETase has PET-degradation activity. The work will be used for development of a new Escherichia coli strain capable of degrading and assimilating PET in its culture medium	Ideonella sakaiensis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Ideonella sakaiensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Ideonella sakaiensis	-	-

Organism

Organism	UniProt	Comment	Textmining
Ideonella sakaiensis	A0A0K8P6T7	-	-
Ideonella sakaiensis 201-F6	A0A0K8P6T7	-	-

Synonyms

Synonyms	Comment	Organism
PETase	-	Ideonella sakaiensis

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Release 2023.1 (January 2023)