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Literature summary for 3.1.1.101 extracted from

  • Wei, R.; Oeser, T.; Barth, M.; Weigl, N.; Lübs, A.; Schulz-Siegmund, M.; Hacker, M.; Zimmermann, W.
    Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles (2014), J. Mol. Catal. B, 103, 72-78.
No PubMed abstract available
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Application

Application Comment Organism
analysis determination of enzymatic hydrolysis by measuring the change of intensity of transmitted light due to the scattering effect of PET nanoparticles immobilized in an agarose gel Thermobifida fusca
degradation biodegradability of PET is mainly influenced by the mobility of the polyester chains, which determine the affinity and accessibility of the ester bonds to the enzyme. The hydrolysis rates of enzymatic PET degradation are predominantly controlled by the efficient substrate adsorption rather than by the hydrolysis of the ester bonds. Nanoparticles prepared from PET samples of different crystallinity show a high proportion of amorphous domains and thus in the corresponding biodegradability Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca E5BBQ3 cf. EC 3.1.1.74
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Thermobifida fusca KW3 E5BBQ3 cf. EC 3.1.1.74
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ethylene terephthalate)n + H2O
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 Thermobifida fusca (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
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 ?
(ethylene terephthalate)n + H2O
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 Thermobifida fusca KW3 (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 ?
additional information analysis of the partially hydrolyzed PET nanoparticles provides indirect evidence for an endo-type hydrolytic mechanism of Cut2 in the heterogeneous degradation of aromatic polyesters Thermobifida fusca ?
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 ?
additional information analysis of the partially hydrolyzed PET nanoparticles provides indirect evidence for an endo-type hydrolytic mechanism of Cut2 in the heterogeneous degradation of aromatic polyesters Thermobifida fusca KW3 ?
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 ?

Synonyms

Synonyms Comment Organism
Cut2
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 Thermobifida fusca