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Literature summary for 3.1.1.101 extracted from

  • Schmidt, J.; Wei, R.; Oeser, T.; Belisario-Ferrari, M.R.; Barth, M.; Then, J.; Zimmermann, W.
    Effect of Tris, MOPS, and phosphate buffers on the hydrolysis of polyethylene terephthalate films by polyester hydrolases (2016), FEBS open bio, 6, 919-927.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.101

Inhibitors

Inhibitors Comment Organism Structure
MOPS hydrolytic activity against PET films is strongly influenced by the reaction medium buffers tris(hydroxymethyl)aminomethane (Tris), 3-(N-morpholino)propanesulfonic acid (MOPS), and sodium phosphate. MOPS acts as a competitive inhibitor Thermobifida fusca
MOPS hydrolytic activity against PET films is strongly influenced by the reaction medium buffers tris(hydroxymethyl)aminomethane (Tris), 3-(N-morpholino)propanesulfonic acid (MOPS), and sodium phosphate. At a MOPS concentration of 1 M, the hydrolysis rate decreases by 90%. MOPS acts as a competitive inhibitor uncultured bacterium
Tris hydrolytic activity against PET films is strongly influenced by the reaction medium buffers tris(hydroxymethyl)aminomethane (Tris), 3-(N-morpholino)propanesulfonic acid (MOPS), and sodium phosphate. At a Tris concentration of 1 M, the hydrolysis rate decreases by about 80%. Tris acts as a competitive inhibitor Thermobifida fusca
Tris hydrolytic activity against PET films is strongly influenced by the reaction medium buffers tris(hydroxymethyl)aminomethane (Tris), 3-(N-morpholino)propanesulfonic acid (MOPS), and sodium phosphate. At a Tris concentration of 1 M, the hydrolysis rate decreases by more than 90%. Tris acts as a competitive inhibitor uncultured bacterium

Organism

Organism UniProt Comment Textmining
Thermobifida fusca E5BBQ3 cf. EC 3.1.1.74
-
Thermobifida fusca KW3 E5BBQ3 cf. EC 3.1.1.74
-
uncultured bacterium G9BY57
-
-

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
80
-
 MOPS pH 8.0, 60°C Thermobifida fusca
170
-
 MOPS pH 8.0, 60°C uncultured bacterium
240
-
 Tris pH 8.0, 60°C uncultured bacterium
440
-
 Tris pH 8.0, 60°C Thermobifida fusca