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Literature summary for 3.1.1.101 extracted from

  • Then, J.; Wei, R.; Oeser, T.; Gerdts, A.; Schmidt, J.; Barth, M.; Zimmermann, W.
    A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate (2016), FEBS open bio, 6, 425-432.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.101

Application

Application Comment Organism
degradation mutant D204C/E253C/D174R causes a weight loss of PET films of 25.0% at 70°C after a reaction time of 48 h, compared to 0.3% for wild-type Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
D174C/D253C increase in melting temperature in absence and in presence of Ca2+ Thermobifida fusca
D204C/E253C increase in melting temperature Thermobifida fusca
D204C/E253C/D174R increase in temperature optimum to 75-80°C, mutant causes a weight loss of PET films of 25.0% at 70 °C after a reaction time of 48 h, compared to 0.3% for wild-type Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca E5BBQ3 cf. EC 3.1.1.74
-
Thermobifida fusca KW3 E5BBQ3 cf. EC 3.1.1.74
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65 70 wild-type Thermobifida fusca
75 80 mutant D204C/E253C/D174R Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
69.8
-
 melting temperature, wild-type Thermobifida fusca
84.6
-
 melting temperature, wild-type, presence of 10 mM Ca2+ Thermobifida fusca
88.3
-
 melting temperature, mutant D174C/E253C Thermobifida fusca
88.3
-
 melting temperature, mutant D204C/E253C Thermobifida fusca
91.6
-
 melting temperature, mutant D174C/E253C, presence of 10 mM Ca2+ Thermobifida fusca