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Literature summary for 3.1.1.101 extracted from

  • Wei, R.; Oeser, T.; Billig, S.; Zimmermann, W.
    A high-throughput assay for enzymatic polyester hydrolysis activity by fluorimetric detection (2012), Biotechnol. J., 7, 1517-1521.
    View publication on PubMed

Application

Application Comment Organism
analysis fluorimetric assay for the fast determination of the activity of polyester-hydrolyzing enzymes in a large number of samples. The assay is robust at different buffer concentrations, reaction times, pH values, and in the presence of proteins and can be used to quantify the amount of terephthalate obtained as the final degradation product of the enzymatic hydrolysis of PET in a microplate format Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca E5BBQ3 cf. EC 3.1.1.74
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Thermobifida fusca KW3 E5BBQ3 cf. EC 3.1.1.74
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