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Literature summary for 3.1.1.101 extracted from

  • Then, J.; Wei, R.; Oeser, T.; Barth, M.; Belisario-Ferrari, M.R.; Schmidt, J.; Zimmermann, W.
    Ca2+ and Mg2+ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca (2015), Biotechnol. J., 10, 592-598.
    View publication on PubMed
Search for more literature for 3.1.1.101

Application

Application Comment Organism
degradation the thermostability of the polyester hydrolase is sufficient to degrade semi-crystalline PET films at 65°C in the presence of 10 mM Ca2+ and 10 mM Mg2+ resulting in weight losses of up to 12.9% after a reaction time of 48 h Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
D204R mutation in residue involved in metal ion binding. Increase in melting point by 14 degrees compared to wild-type. Presence of 10 mM CaCl2 does not result in a considerable increase in melting point Thermobifida fusca
E253R mutation in residue involved in metal ion binding. Increase in melting point by 14 degrees compared to wild-type. Presence of 10 mM CaCl2 does not result in a considerable increase in melting point Thermobifida fusca

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ and Mg2+, presence of 10 mM each increases thermostability by about 10 degrees. Residues Asp174, Asp204, and Glu253 are potential binding residues for the two cations Thermobifida fusca
Ca2+ and Mg2+, presence of 10 mM each increases thermostability by about 14 degrees Thermobifida fusca
Mg2+ and Ca2+, presence of 10 mM each increases thermostability by about 10 degrees Thermobifida fusca
Mg2+ and Ca2+, presence of 10 mM each increases thermostability by about 10 degrees. Residues Asp174, Asp204, and Glu253 are potential binding residues for the two cations Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca E5BBQ2 cf. EC 3.1.1.74
-
Thermobifida fusca E5BBQ3
-
-
Thermobifida fusca KW3 E5BBQ2 cf. EC 3.1.1.74
-
Thermobifida fusca KW3 E5BBQ3
-
-

Synonyms

Synonyms Comment Organism
Cut1
-
 Thermobifida fusca
cut_1.KW3
-
 Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the addition of 10 mM Ca2+ or Mg2+ cations to the enzyme results in an increase of the melting point of about 10°C Thermobifida fusca
additional information
-
 the addition of 10 mM Ca2+ or Mg2+ cations to the enzyme results in an increase of the melting point of up to 14 degrees Thermobifida fusca
71.2
-
 melting point Thermobifida fusca