Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.101 extracted from

  • Wei, R.; Oeser, T.; Then, J.; Kühn, N.; Barth, M.; Schmidt, J.; Zimmermann, W.
    Functional characterization and structural modeling of synthetic polyester-degrading hydrolases from Thermomonospora curvata (2014), AMB Express, 4, 1-10.
    View publication on PubMed
Search for more literature for 3.1.1.101

Application

Application Comment Organism
degradation at 50°C, a maximum hydrolysis rate for poly(ethylene terephthalate) nanoparticles of 0.0033 per min is determined with 80 microg/ml of Tcur_1278. With 50 microg/ml of Tcur_1278, the hydrolysis rate increases 1.8fold at 55°C and 2.6fold at 60°C Thermomonospora curvata
degradation at 50°C, a maximum hydrolysis rate of poly(ethylene terephthalate) nanoparticles of 0.0059 per min is determined with 20 microg/ml of Tcur_0390 Thermomonospora curvata

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermomonospora curvata

Crystallization (Commentary)

Crystallization (Comment) Organism
structural model generated on the basis of PDB ID 3VIS. The polyester hydrolase reveals a typical alpha/beta hydrolase fold. The catalytic triad formed by residues S130, D176 and H208 is exposed to the solvent Thermomonospora curvata

Organism

Organism UniProt Comment Textmining
Thermomonospora curvata D1A2H1 cf. EC 3.1.1.3
-
Thermomonospora curvata D1A9G5 cf. EC 3.1.1.3
-
Thermomonospora curvata DSM 43183 D1A2H1 cf. EC 3.1.1.3
-
Thermomonospora curvata DSM 43183 D1A9G5 cf. EC 3.1.1.3
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83.1
-
 pH 8.5, 25°C Thermomonospora curvata
88.8
-
 pH 8.5, 25°C Thermomonospora curvata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(epsilon-caprolactone)n + H2O
-
 Thermomonospora curvata (epsilon-caprolactame)n-1 + ethylene terephthalate
-
 ?
(epsilon-caprolactone)n + H2O
-
 Thermomonospora curvata DSM 43183 (epsilon-caprolactame)n-1 + ethylene terephthalate
-
 ?
(ethylene terephthalate)n + H2O
-
 Thermomonospora curvata (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 ?
(ethylene terephthalate)n + H2O
-
 Thermomonospora curvata DSM 43183 (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 ?
4-nitrophenyl butanoate + H2O
-
 Thermomonospora curvata 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl butanoate + H2O
-
 Thermomonospora curvata DSM 43183 4-nitrophenol + butanoate
-
 ?

Synonyms

Synonyms Comment Organism
Tcur_0390
-
 Thermomonospora curvata
Tcur_1278
-
 Thermomonospora curvata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermomonospora curvata
60
-
-
 Thermomonospora curvata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 60 min, 40% residual activity Thermomonospora curvata
55
-
 60 min, 80% residual activity Thermomonospora curvata
60
-
 10 min, 15% residual activity Thermomonospora curvata
60
-
 10 min, 35% residual activity Thermomonospora curvata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
 4-nitrophenyl butanoate pH 8.5, 25°C Thermomonospora curvata
12.4
-
 4-nitrophenyl butanoate pH 8.5, 25°C Thermomonospora curvata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Thermomonospora curvata

pH Range

pH Minimum pH Maximum Comment Organism
9.5
-
 60% of maximum activity Thermomonospora curvata