Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1% v/v, 141.5% activity | Geobacillus thermoleovorans | |
EDTA | 1 mM, 143.2% activity | Geobacillus thermoleovorans | |
Triton X-100 | 1% v/v, 136.9% activity | Geobacillus thermoleovorans | |
Tween 20 | 1% v/v, 121.9% activity | Geobacillus thermoleovorans |
Cloned (Comment) | Organism |
---|---|
gene caesCCR11, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3) | Geobacillus thermoleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | - |
Geobacillus thermoleovorans | |
butanol | - |
Geobacillus thermoleovorans | |
dioxane | - |
Geobacillus thermoleovorans | |
ethanol | - |
Geobacillus thermoleovorans | |
ethyl acetate | - |
Geobacillus thermoleovorans | |
heptane | - |
Geobacillus thermoleovorans | |
hexane | - |
Geobacillus thermoleovorans | |
Hg2+ | 90% inhibition | Geobacillus thermoleovorans | |
K+ | 10% inhibition | Geobacillus thermoleovorans | |
methanol | - |
Geobacillus thermoleovorans | |
PMSF | 1 mM, 85% inhibition | Geobacillus thermoleovorans | |
Propanol | - |
Geobacillus thermoleovorans | |
SDS | 0.1% w/v, 24.2% inhibition | Geobacillus thermoleovorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geobacillus thermoleovorans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates slightly | Geobacillus thermoleovorans | |
Li+ | activates slightly | Geobacillus thermoleovorans | |
Mg2+ | activates slightly | Geobacillus thermoleovorans | |
Na+ | activates slightly | Geobacillus thermoleovorans |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 21.8% residual activity after 1 h | Geobacillus thermoleovorans |
Butanol | 22.9% residual activity after 1 h | Geobacillus thermoleovorans |
dioxane | 13.2% residual activity after 1 h | Geobacillus thermoleovorans |
Ethanol | 27.5% residual activity after 1 h | Geobacillus thermoleovorans |
Ethyl acetate | 24.8% residual activity after 1 h | Geobacillus thermoleovorans |
heptane | 74.6% residual activity after 1 h | Geobacillus thermoleovorans |
hexane | 59.2% residual activity after 1 h | Geobacillus thermoleovorans |
Methanol | 12.7% residual activity after 1 h | Geobacillus thermoleovorans |
propanol | 13.1% residual activity after 1 h | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | W8QMJ2 | isolated from El Carrizal hot springs in Veracruz, Mexico | - |
Geobacillus thermoleovorans CCR11 | W8QMJ2 | isolated from El Carrizal hot springs in Veracruz, Mexico | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme and recombinant enzyme by ultrafiltration | Geobacillus thermoleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1375.7 | - |
purified recombinant enzyme, pH 6.5, 30°C, substrate 4-nitrophenyl laurate | Geobacillus thermoleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | best substrate | Geobacillus thermoleovorans | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | best substrate | Geobacillus thermoleovorans CCR11 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Geobacillus thermoleovorans CCR11 | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Geobacillus thermoleovorans CCR11 | 4-nitrophenol + caproate | - |
? | |
additional information | enzyme CaesCCR11 has a preference for C4 substrates. Activity toward 4-nitrophenyl esters with longer chain lengths is only slightly detected, suggesting that CaesCCR11 is an esterase | Geobacillus thermoleovorans | ? | - |
? | |
additional information | enzyme CaesCCR11 has a preference for C4 substrates. Activity toward 4-nitrophenyl esters with longer chain lengths is only slightly detected, suggesting that CaesCCR11 is an esterase | Geobacillus thermoleovorans CCR11 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27300, about, sequence calculation | Geobacillus thermoleovorans |
Synonyms | Comment | Organism |
---|---|---|
CaesCCR11 | - |
Geobacillus thermoleovorans |
More | cf. EC 3.1.1.3 | Geobacillus thermoleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | purified recombinant enzyme, at 30°C and 40°C, lipolytic activity is conserved after 30 and 20 min, respectively. When the enzyme is preincubated around the physiological temperature at 50-60°C for 30 min, lipolytic activity is increased by 80%, it is diminished by 50% at 70°C and completely lost at 80°C after 10 min | Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | broad optimum, recombinant enzyme | Geobacillus thermoleovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | purified recombinant enzyme, the activity is retained. Lipolytic activity is increased to 150% after a 28 h incubation at pH 9.0 and pH 10.0, while activity is lost by 50% at pH 5.0 and pH 11.0 | Geobacillus thermoleovorans |
General Information | Comment | Organism |
---|---|---|
evolution | CaesCCR11 as a member of family XV of lipolytic enzymes, it has a canonical alpha/beta hydrolase fold composed of 7 beta-strands and 6 alpha-helices, the alpha/beta architecture of the cap domain, the GLSTG pentapeptide, and the formation of distinctive salt bridges | Geobacillus thermoleovorans |
additional information | three-dimensional structure modeling of enzyme CaesCCR11 using the Bacillus sp. monoacyl glycerol lipase bMGL H-257 structure as template (PBD ID 3RM3) | Geobacillus thermoleovorans |