Literature summary for 3.1.1.1 extracted from

Yang, X.; Dai, J.; Zhao, S.; Li, R.; Goulette, T.; Chen, X.; Xiao, H.
Identification and characterization of a novel carboxylesterase from Phaseolus vulgaris for detection of organophosphate and carbamates pesticides (2018), J. Sci. Food. Agric., 98, 5095-5104 .

Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
sequence comparisons	Phaseolus vulgaris

Inhibitors

Inhibitors	Comment	Organism	Structure
Aldicarb	-	Phaseolus vulgaris
bis-4-nitrophenyl phosphate	BNPP, a specific carboxylesterase inhibitor, strong inhibition at 1 mM	Phaseolus vulgaris
carbofuran	-	Phaseolus vulgaris
chlorpyrifos	-	Phaseolus vulgaris
dichlorvos	-	Phaseolus vulgaris
Dimethoate	-	Phaseolus vulgaris
donepezil hydrochloride	-	Phaseolus vulgaris
glufosinate	low to moderate inhibition	Phaseolus vulgaris
methomyl	-	Phaseolus vulgaris
Monocrotophos	-	Phaseolus vulgaris
additional information	kidney bean esterase can be inhibited by organophosphate and carbamate pesticides, which can be substituted for acetylcholinesterase, sensitivity and specificity of kidney bean esterase on pesticides, inhibition kinetics, overview. The enzyme inhibition rate decreases in the order BNPP, physostigmine, donepezil hydrochloride, and the inhibition rate of BNPP is significantly higher than that of the latter two at 1 mM	Phaseolus vulgaris
omethoate	-	Phaseolus vulgaris
phoxim	-	Phaseolus vulgaris
physostigmine	-	Phaseolus vulgaris
propoxur	-	Phaseolus vulgaris
triazophos	-	Phaseolus vulgaris
Trichlorfon	-	Phaseolus vulgaris

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics	Phaseolus vulgaris

Organism

Organism	UniProt	Comment	Textmining
Phaseolus vulgaris	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from crude kidney bean powder extract 56.2fold by anion exchange chromatography, ultrafiltration, and gel filtration	Phaseolus vulgaris

Reaction

Reaction	Comment	Organism	Reaction ID
a carboxylic ester + H2O = an alcohol + a carboxylate	the carboxylesterases catalytic apparatus typically involves three residues in a catalytic triad: a cysteine or serine, a glutamate or aspartate and a histidine, and often the mechanism involves a nucleophilic attack on a carbonyl carbon atom	Phaseolus vulgaris	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	kidney bean	Phaseolus vulgaris	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.843	-	purified native enzyme, pH 6.5, 35°C, substrate 2-naphthyl acetate	Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-naphthyl acetate + H2O	-	Phaseolus vulgaris	2-naphthol + acetate	-	?
S-acetylthiocholine iodide + H2O	low activity	Phaseolus vulgaris	thiocholine iodide + acetate	-	?
S-butyrylthiocholine iodide + H2O	low activity	Phaseolus vulgaris	thiocholine iodide + butyrate	-	?

Synonyms

Synonyms	Comment	Organism
kidney bean esterase	-	Phaseolus vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Phaseolus vulgaris

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	over 50% of maximal activity within this range	Phaseolus vulgaris

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Phaseolus vulgaris

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	7.5	over 60% of maximal activity within this range	Phaseolus vulgaris

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Release 2023.1 (January 2023)