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Literature summary for 3.1.1.1 extracted from

  • Fucinos, P.; Pastrana, L.; Sanroman, A.; Longo, M.; Hermoso, J.; Rua, M.
    An esterase from Thermus thermophilus HB27 with hyper-thermoalkalophilic properties Purification, characterisation and structural modelling (2011), J. Mol. Catal. B, 70, 127-137 .
No PubMed abstract available
Search for more literature for 3.1.1.1

Application

Application Comment Organism
biotechnology due to its high thermal stability the enzyme is a very attractive enzyme for biotechnological purposes Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-associated enzyme Thermus thermophilus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Thermus thermophilus
36445
-
 calculated from sequence Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72J75
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
317.2
-
 65°C, pH 8.0 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl decanoate + H2O preference for medium chain-length esters Thermus thermophilus 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl dodecanoate + H2O preference for medium chain-length esters Thermus thermophilus 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl tetradecanoate + H2O preference for medium chain-length esters Thermus thermophilus 4-nitrophenol + tetradecanoate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
E34Tt
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 above Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 half-life: 135 min Thermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermus thermophilus calculated from sequence
-
 8.37