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Literature summary for 3.1.1.1 extracted from

  • Liu, C.; Yang, G.; Wu, L.; Tian, G.; Zhang, Z.; Feng, Y.
    Switch of substrate specificity of hyperthermophilic acylaminoacyl peptidase by combination of protein and solvent engineering (2011), Protein Cell, 2, 497-506.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
F488G/R526V/T560W 1.55fold increase in activity with 4-nitrophenyl dodecanoate compared to activity of mutant R526V/T560W Aeropyrum pernix
additional information the esterase activity of the mutant R526V (this mutation transforms a promiscuous acylaminoacyl peptidase into a specific carboxylesterase) towards substrates with long acyl chains is enhanced by protein engineering and solvent optimization. The substrate preference of the enzyme can be further changed from 4-nitrophenyl octanoate to 4-nitrophenyl dodecanoate by protein and solvent engineering Aeropyrum pernix
R526V mutant enzyme with high esterase activity, extreme thermal stability, and high tolerance to organic solvents Aeropyrum pernix
R526V/T560W 1.5fold increase in activity with 4-nitrophenyl dodecanoate compared to activity of mutant R526V Aeropyrum pernix
W474V/F488G/R526V/T560W the mutant enzyme has 7fold higher catalytic efficiency (kcat/Km) for 4-nitrophenyl dodecanoate than the mutant enzyme R526V Aeropyrum pernix
W474V/R526V/T560W 3.11fold increase in activity with 4-nitrophenyl dodecanoate compared to activity of mutant R526V/T560W Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00075
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474Q/R526V/T560W Aeropyrum pernix
0.00076
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488Y/R526V/T560W Aeropyrum pernix
0.00101
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488W/R526V/T560W Aeropyrum pernix
0.00122
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme R526V/T560W Aeropyrum pernix
0.00183
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474A/R526V/T560W Aeropyrum pernix
0.00234
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474V/F488G/R526V/T560W Aeropyrum pernix
0.00243
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474V/R526V/T560W Aeropyrum pernix
0.00388
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488P/R526V/T560W Aeropyrum pernix
0.00579
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488S/R526V/T560W Aeropyrum pernix
0.00737
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488A/R526V/T560W Aeropyrum pernix
0.00834
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488G/R526V/T560W Aeropyrum pernix
0.00862
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, wild-type enzyme Aeropyrum pernix
0.0118
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme R526V Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YBQ2
-
-
Aeropyrum pernix DSM 11879 Q9YBQ2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl dodecanoate + H2O switch of substrate specificity of hyperthermophilic promiscuous acylaminoacyl peptidase by combination of protein and solvent engineering into a specific carboxylesterase Aeropyrum pernix 4-nitrophenol + dodecanoate
-
?
4-nitrophenyl dodecanoate + H2O switch of substrate specificity of hyperthermophilic promiscuous acylaminoacyl peptidase by combination of protein and solvent engineering into a specific carboxylesterase Aeropyrum pernix DSM 11879 4-nitrophenol + dodecanoate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.35
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, wild-type enzyme Aeropyrum pernix
1.73
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488W/R526V/T560W Aeropyrum pernix
2.05
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488Y/R526V/T560W Aeropyrum pernix
5.29
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474Q/R526V/T560W Aeropyrum pernix
5.47
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme R526V Aeropyrum pernix
7.96
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme R526V/T560W Aeropyrum pernix
8.17
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488P/R526V/T560W Aeropyrum pernix
10.45
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488S/R526V/T560W Aeropyrum pernix
12.08
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488A/R526V/T560W Aeropyrum pernix
12.61
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474A/R526V/T560W Aeropyrum pernix
17.17
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488G/R526V/T560W Aeropyrum pernix
23.5
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474V/R526V/T560W Aeropyrum pernix
26.25
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474V/F488G/R526V/T560W Aeropyrum pernix

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
157.2
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, wild-type enzyme Aeropyrum pernix
462.5
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme R526V Aeropyrum pernix
1638
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488A/R526V/T560W Aeropyrum pernix
1712
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488W/R526V/T560W Aeropyrum pernix
1804
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488S/R526V/T560W Aeropyrum pernix
2059
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488G/R526V/T560W Aeropyrum pernix
2107
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488P/R526V/T560W Aeropyrum pernix
2703
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme F488Y/R526V/T560W Aeropyrum pernix
6528
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme R526V/T560W Aeropyrum pernix
6876
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474A/R526V/T560W Aeropyrum pernix
7018
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474Q/R526V/T560W Aeropyrum pernix
9662
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474V/R526V/T560W Aeropyrum pernix
11220
-
4-nitrophenyl dodecanoate pH 8.0, 60°C, mutant enzyme W474V/F488G/R526V/T560W Aeropyrum pernix