Application | Comment | Organism |
---|---|---|
synthesis | synthesis of (S)-alpha-arylpropionates (profens). The reaction proceeds via enantioselective hydrolysis of the malonic acid ester to the monoester. Cleavage of the carboxyl group at the active site of the enzyme leads to the formation of the prochiral intermediate, which is then protonated to give the enantioenriched monoacid. Engineering of the active site gives rise to a variant with good stereoselectivity. As the esterase-catalyzed domino reaction does not need external cofactors and allows a theoretical yield of 100% | Sulfurisphaera tokodaii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
A179K | increased stereoselectivity in synthesis of naproxen compared to the wild-type enzyme, (S)-selective, 64% enantioneric excess (compared to wild-type value 21% enantiomeric excess) | Sulfurisphaera tokodaii |
A179M/198E | increased stereoselectivity in synthesis of naproxen compared to the wild-type enzyme, (S)-selective, 18% enantioneric excess (compared to wild-type value 21% enantiomeric excess) | Sulfurisphaera tokodaii |
A179M/198Y | increased stereoselectivity in synthesis of naproxen compared to the wild-type enzyme, (S)-selective, 31% enantioneric excess (compared to wild-type value 21% enantiomeric excess) | Sulfurisphaera tokodaii |
I203E | increased stereoselectivity in synthesis of naproxen compared to the wild-type enzyme, (S)-selective, 52% enantioneric excess (compared to wild-type value 21% enantiomeric excess) | Sulfurisphaera tokodaii |
I203R | increased stereoselectivity in synthesis of naproxen compared to the wild-type enzyme, (S)-selective, 63% enantioneric excess (compared to wild-type value 21% enantiomeric excess) | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q976W8 | - |
- |
Sulfurisphaera tokodaii 7 | Q976W8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Sulfurisphaera tokodaii | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Sulfurisphaera tokodaii 7 | 4-nitrophenol + butyrate | - |
? | |
diethyl (6-methoxynaphthalen-2-yl)(methyl)propanedioate + H2O | - |
Sulfurisphaera tokodaii | ethanol + (2S)-3-ethoxy-2-(6-methoxynaphthalen-2-yl)-2-methyl-3-oxopropanoic acid | - |
? | |
diethyl (6-methoxynaphthalen-2-yl)(methyl)propanedioate + H2O | - |
Sulfurisphaera tokodaii 7 | ethanol + (2S)-3-ethoxy-2-(6-methoxynaphthalen-2-yl)-2-methyl-3-oxopropanoic acid | - |
? | |
diethyl methyl(phenyl)propanedioate + H2O | - |
Sulfurisphaera tokodaii | ethanol + (2S)-3-ethoxy-2-methyl-3-oxo-2-phenylpropanoic acid | - |
? | |
diethyl methyl(phenyl)propanedioate + H2O | - |
Sulfurisphaera tokodaii 7 | ethanol + (2S)-3-ethoxy-2-methyl-3-oxo-2-phenylpropanoic acid | - |
? | |
ethyl (2S)-2-(6-methoxynaphthalen-2-yl)propanoate + H2O | - |
Sulfurisphaera tokodaii | ethanol + (2S)-2-(6-methoxynaphthalen-2-yl)propanoate | - |
? | |
ethyl (2S)-2-(6-methoxynaphthalen-2-yl)propanoate + H2O | - |
Sulfurisphaera tokodaii 7 | ethanol + (2S)-2-(6-methoxynaphthalen-2-yl)propanoate | - |
? | |
ethyl (2S)-2-phenylpropanoate + H2O | - |
Sulfurisphaera tokodaii | ethanol + (2S)-2-phenylpropanoate | - |
? | |
ethyl (2S)-2-phenylpropanoate + H2O | - |
Sulfurisphaera tokodaii 7 | ethanol + (2S)-2-phenylpropanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Est0071 | - |
Sulfurisphaera tokodaii |
ST0071 | locus name | Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Sulfurisphaera tokodaii |