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Literature summary for 3.1.1.1 extracted from

  • Weia, T.; Fenga, S.; Shenb, Y.; Hea, P.; Maa, G.; Yua, X.; Zhang, F.; Mao, D.
    Characterization of a novel thermophilic pyrethroid-hydrolyzing carboxylesterase from Sulfolobus tokodaii into a new family (2013), J. Mol. Catal. B, 97, 225-232.
No PubMed abstract available
Search for more literature for 3.1.1.1

Application

Application Comment Organism
degradation the enzyme can efficiently hydrolyze a wide range of synthetic pyrethroids including fenpropathrin, permethrin, cypermethrin, cyhalothrin, deltamethrin and bifenthrin, which makes it a potential candidate for the detoxification of pyrethroids for the purpose of biodegradation Sulfurisphaera tokodaii
environmental protection the enzyme can efficiently hydrolyze a wide range of synthetic pyrethroids including fenpropathrin, permethrin, cypermethrin, cyhalothrin, deltamethrin and bifenthrin, which makes it a potential candidate for the detoxification of pyrethroids for the purpose of biodegradation Sulfurisphaera tokodaii

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
acetone 50% (v/v), 2 h incubation, 60% loss of activity Sulfurisphaera tokodaii
chloroform 50% (v/v), 2 h incubation, 66% loss of activity Sulfurisphaera tokodaii
Co2+ 5 mM, 38% inhibition Sulfurisphaera tokodaii
Cu2+ 5 mM, 57% inhibition; 5 mM, reduces the esterase activity to 43% Sulfurisphaera tokodaii
diethyldicarbonate 5 mM,% inhibition Sulfurisphaera tokodaii
diethylpyrocarbonate 5 mM,% inhibition Sulfurisphaera tokodaii
dimethylformamide 50% (v/v), 2 h incubation, 72% loss of activity Sulfurisphaera tokodaii
DTT 5 mM,% inhibition Sulfurisphaera tokodaii
EDTA 5 mM, 17% inhibition Sulfurisphaera tokodaii
ethanol 50% (v/v), 2 h incubation, 67% loss of activity Sulfurisphaera tokodaii
Fe2+ 5 mM, 81% inhibition; 5 mM, reduces the esterase activity to 19% Sulfurisphaera tokodaii
formaldehyde 20% (v/v), 2 h incubation, 81% loss of activity Sulfurisphaera tokodaii
heptane 20% (v/v), 2 h incubation, 88% loss of activity Sulfurisphaera tokodaii
methanol 50% (v/v), 2 h incubation, 58% loss of activity Sulfurisphaera tokodaii
Mn2+ 5 mM, 27% inhibition Sulfurisphaera tokodaii
n-hexane 20% (v/v), 2 h incubation, 67% loss of activity Sulfurisphaera tokodaii
Ni2+ 5 mM, 22% inhibition Sulfurisphaera tokodaii
PMSF 5 mM, complete inhibition Sulfurisphaera tokodaii
Propanol 50% (v/v), 2 h incubation, 59% loss of activity Sulfurisphaera tokodaii
SDS 5% (w/v), 90% inhibition Sulfurisphaera tokodaii
Toluene 20% (v/v), 2 h incubation, 86% loss of activity Sulfurisphaera tokodaii
Triton X-100 5% (w/v), 42% inhibition Sulfurisphaera tokodaii
Tween 20 5% (w/v), 28% inhibition Sulfurisphaera tokodaii
Tween 80 5% (w/v), 38% inhibition Sulfurisphaera tokodaii
Zn2+ 5 mM, 87% inhibition; 5 mM, reduces the esterase activity to 13% Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 4-nitrophenyl hexadecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.0051
-
 4-nitrophenyl hexadecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.009
-
 4-nitrophenyl tetradecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.0094
-
 4-nitrophenyl tetradecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.015
-
 4-nitrophenyl dodecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.033
-
 4-nitrophenyl decanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.152
-
 4-nitrophenyl octanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.153
-
 4-nitrophenyl octanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.239
-
 4-nitrophenyl butyrate pH 9.0, 80°C Sulfurisphaera tokodaii
0.464
-
 4-nitrophenyl acetate pH 9.0, 80°C Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information 5 mM of various metal ions (Ca2+, Mg2+, Mn2+, Co2+,Ni2+, Na+, and K+) do not significantly affect the enzymatic activity after incubation for 60 min at room temperature Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Sulfurisphaera tokodaii
37400
-
 x * 37400, calculated from sequence Sulfurisphaera tokodaii

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 50% (v/v), 2 h incubation, 60% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
Acetone 50% (v/v), pH 9.0, 30°C, 60% loss of activity Sulfurisphaera tokodaii
chloroform 50% (v/v), 2 h incubation, 66% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
chloroform 50% (v/v), pH 9.0, 30°C, 66% loss of activity Sulfurisphaera tokodaii
dimethyl formamide 50% (v/v), 2 h incubation, 72% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
dimethylformamide 50% (v/v), pH 9.0, 30°C, 72% loss of activity Sulfurisphaera tokodaii
Ethanol 50% (v/v), 2 h incubation, 67% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
Ethanol 50% (v/v), pH 9.0, 30°C, 67% loss of activity Sulfurisphaera tokodaii
formaldehyde 20% (v/v), 2 h incubation, 81% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
formaldehyde 50% (v/v), pH 9.0, 30°C, 81% loss of activity Sulfurisphaera tokodaii
heptane 20% (v/v), 2 h incubation, 88% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
heptane 50% (v/v), pH 9.0, 30°C, 88% loss of activity Sulfurisphaera tokodaii
Methanol 50% (v/v), 2 h incubation, 58% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
Methanol 50% (v/v), pH 9.0, 30°C, 58% loss of activity Sulfurisphaera tokodaii
n-hexane 20% (v/v), 2 h incubation, 67% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
n-hexane 50% (v/v), pH 9.0, 30°C, 77% loss of activity Sulfurisphaera tokodaii
propanol 50% (v/v), 2 h incubation, 59% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
propanol 50% (v/v), pH 9.0, 30°C, 59% loss of activity Sulfurisphaera tokodaii
toluene 20% (v/v), 2 h incubation, 86% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
toluene 50% (v/v), pH 9.0, 30°C, 86% loss of activity Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q96Z02
-
-
Sulfurisphaera tokodaii 7 Q96Z02
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
 substrate: 4-nitrophenyl hexadecanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
19
-
 substrate: 4-nitrophenyl tetradecanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
37
-
 substrate: 4-nitrophenyl dodecanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
56
-
 substrate: 4-nitrophenyl decanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
189
-
 substrate: 4-nitrophenyl octanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
213
-
 substrate: 4-nitrophenyl acetate, pH 9.0, 80°C Sulfurisphaera tokodaii
354
-
 substrate: 4-nitrophenyl butyrate, pH 9.0, 80°C Sulfurisphaera tokodaii
3542
-
 pH 9.0, 80°C, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Sulfurisphaera tokodaii 7 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O most preferable substrate Sulfurisphaera tokodaii 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O most preferable substrate Sulfurisphaera tokodaii 7 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl decanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl decanoate + H2O
-
 Sulfurisphaera tokodaii 7 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl dodecanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl dodecanoate + H2O
-
 Sulfurisphaera tokodaii 7 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl hexadecanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + hexadecanoate
-
 ?
4-nitrophenyl octanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl octanoate + H2O
-
 Sulfurisphaera tokodaii 7 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl tetradecanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + tetradecanoate
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Sulfurisphaera tokodaii
? x * 37400, calculated from sequence Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
EstSt7
-
 Sulfurisphaera tokodaii
ST2026
-
 Sulfurisphaera tokodaii
ST2026 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Sulfurisphaera tokodaii
80
-
 substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 95 50°C: about 40% of maximal activity, 95°C: about 50% of maximal activity Sulfurisphaera tokodaii
50 95 50°C: about 40% of maximal activity, 95°C: about 50% of maximal activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable for at least 240 min Sulfurisphaera tokodaii
90
-
 half-life: 180 min Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 4-nitrophenyl hexadecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.11
-
 4-nitrophenyl tetradecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
0.2
-
 4-nitrophenyl dodecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
1.5
-
 4-nitrophenyl decanoate pH 9.0, 80°C Sulfurisphaera tokodaii
21.1
-
 4-nitrophenyl octanoate pH 9.0, 80°C Sulfurisphaera tokodaii
58.9
-
 4-nitrophenyl butyrate pH 9.0, 80°C Sulfurisphaera tokodaii
74.6
-
 4-nitrophenyl acetate pH 9.0, 80°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Sulfurisphaera tokodaii
9
-
 substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
7 11 pH 7.0: about 40% of maximal activity, pH 11: about 40% of maximal activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 80°C, 60 min, 80% of maximal activity Sulfurisphaera tokodaii
8 10 80°C, 60 min, the enzyme maintains over 80% of its maximal activity Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.9
-
 4-nitrophenyl hexadecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
11.7
-
 4-nitrophenyl tetradecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
13.5
-
 4-nitrophenyl dodecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
45.7
-
 4-nitrophenyl decanoate pH 9.0, 80°C Sulfurisphaera tokodaii
138
-
 4-nitrophenyl octanoate pH 9.0, 80°C Sulfurisphaera tokodaii
160.7
-
 4-nitrophenyl acetate pH 9.0, 80°C Sulfurisphaera tokodaii
246.3
-
 4-nitrophenyl butyrate pH 9.0, 80°C Sulfurisphaera tokodaii