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Literature summary for 3.1.1.1 extracted from
- Exceptional thermal stability and organic solvent tolerance of an esterase expressed from a thermophilic host (2012), Appl. Microbiol. Biotechnol., 93, 1965-1974.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as thioredoxin-free form (EcSisEstA I) and as thioredoxin-EstA fusion protein (EcSisEstA II). Expression in Sulfolobus islandicus E233S, a DELTASpyrEF DELTAlacS mutant constructed from Sulfolobus islandicus REY15A. Expressing the enzyme in a mesophilic host renders the enzyme much less thermoactive | Sulfolobus islandicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 1 mM, 14% loss of activity (recombinant enzyme expressed in Sulfolobus solfataricus). 1 mM, 19% loss of activity (recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I)). 1 mM, 23% loss of activity (recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II)) | Sulfolobus islandicus |  |
| EDTA | 5 mM, 42% loss of activity (recombinant enzyme expressed in Sulfolobus solfataricus). 5 mM, 69% loss of activity (recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I)). 5 mM, 65% loss of activity (recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II)) | Sulfolobus islandicus | |
| mercaptoethanol | 5 mM, 15% loss of activity (recombinant enzyme expressed in Sulfolobus solfataricus). 5 mM, 25% loss of activity (recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I)). 5 mM, 22% loss of activity (recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II)) | Sulfolobus islandicus | |
| PMSF | 1 mM, 79% loss of activity (recombinant enzyme expressed in Sulfolobus solfataricus). 1 mM, 89% loss of activity (recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I)). 1 mM, 81% loss of activity (recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II)) | Sulfolobus islandicus | |
| Urea | 5 mM, 16% loss of activity (recombinant enzyme expressed in Sulfolobus solfataricus). 5 mM, 17% loss of activity (recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I)). 5 mM, 10% loss of activity (recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II)) | Sulfolobus islandicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0191 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 0.0203 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 0.0219 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 0.0239 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 0.0241 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 0.0254 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 0.0331 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 0.0356 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 0.0361 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 0.227 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 0.252 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 0.61 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE, recombinant enzyme expressed in Sulfolobus solfataricus, dimer | Sulfolobus islandicus |
| 67000 | - |
gel filtration, native PAGE, recombinant enzyme expressed in Sulfolobus solfataricus, dimer | Sulfolobus islandicus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 1-butanol | 30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 36% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 18% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 29% residual activity | Sulfolobus islandicus |
| 1-propanol | 30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 76% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 32% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 39% residual activity | Sulfolobus islandicus |
| 2-propanol | 30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 95% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 30% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 35% residual activity | Sulfolobus islandicus |
| Acetone | 30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 91% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 63% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 81% residual activity | Sulfolobus islandicus |
| acetonitrile | 30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 77% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 40% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 50% residual activity | Sulfolobus islandicus |
| chloroform | 30% v/v, complete inactivation of recombinant enzyme expressed in Sulfolobus solfataricus or Escherichia coli | Sulfolobus islandicus |
| DMSO | 30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 98% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 73% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 81% residual activity | Sulfolobus islandicus |
| Ethanol | up to 30% v/v, activity of recombinant enzyme expressed in Sulfolobus solfataricus is not affected or slightly increased. The recombinant enzyme expressed in Escherichia coli shows about 40% residual activity | Sulfolobus islandicus |
| hexane | 30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 36% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 10% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 9% residual activity | Sulfolobus islandicus |
| isoamyl alcohol | 30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 36% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 8% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 9% residual activity | Sulfolobus islandicus |
| Methanol | 30% v/v, activity of recombinant enzyme expressed in Sulfolobus solfataricus is not affected. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 61% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 76% residual activity | Sulfolobus islandicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus islandicus | F0NDQ1 | - |
- |
| Sulfolobus islandicus Rey15A | F0NDQ1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus islandicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butanoate + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl decanoate + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl dodecanoate + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + dodecanoate | - |
? | |
| 4-nitrophenyl dodecanoate + H2O | - |
Sulfolobus islandicus Rey15A | 4-nitrophenol + dodecanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | - |
Sulfolobus islandicus Rey15A | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Sulfolobus islandicus Rey15A | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl pentanoate + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + pentanoate | - |
? | |
| 4-nitrophenyl pentanoate + H2O | - |
Sulfolobus islandicus Rey15A | 4-nitrophenol + pentanoate | - |
? | |
| additional information | recombinant enzyme expressed in Escherichia coli and recombinant enzyme expressed in Sulfolobus solfataricus show the same catalytic properties | Sulfolobus islandicus | ? | - |
? | |
| additional information | recombinant enzyme expressed in Escherichia coli and recombinant enzyme expressed in Sulfolobus solfataricus show the same catalytic properties | Sulfolobus islandicus Rey15A | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, SDS-PAGE, recombinant enzyme expressed in Sulfolobus solfataricus, dimer | Sulfolobus islandicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EstA | - |
Sulfolobus islandicus |
| SiRe0290 | - |
Sulfolobus islandicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
24 h, recombinant enzyme expressed in Sulfolobus solfataricus retains more than 85% of its activity | Sulfolobus islandicus |
| 90 | - |
half-life of recombinant enzyme expressed in Sulfolobus solfataricus: 16 h. Recombinant enzyme expressed in Escherichia coli loses about 50% of its activity | Sulfolobus islandicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 285 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 296 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 508 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 526 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 764 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 803 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 1032 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 1080 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 1124 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 1228 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 1756 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 2003 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1200 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 1300 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 1800 | - |
4-nitrophenyl palmitate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 25900 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 26600 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 30200 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 31200 | - |
4-nitrophenyl decanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 33300 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 47200 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) | Sulfolobus islandicus | |
| 49300 | - |
4-nitrophenyl butanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
| 51300 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) | Sulfolobus islandicus | |
| 60400 | - |
4-nitrophenyl hexanoate | pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus | Sulfolobus islandicus | |
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