Cloned (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
expression in Escherichia coli | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
D163N | total loss of activity | Archaeoglobus fulgidus |
D163N | inactive mutant enzyme | Archaeoglobus fulgidus |
D169N | 45% loss of activity compared to wild-type enzyme | Archaeoglobus fulgidus |
D169N | 50% of wild-type activity remains | Archaeoglobus fulgidus |
H210N | total loss of activity | Archaeoglobus fulgidus |
H210N | 95% loss of activity compared to wild-type enzyme | Archaeoglobus fulgidus |
S136C | total loss of activity | Archaeoglobus fulgidus |
S136C | inactive mutant enzyme | Archaeoglobus fulgidus |
Y244stop | a truncated version of the enzyme covering the 244 N-terminal residues reveals measurable activity | Archaeoglobus fulgidus |
Y255stop | 88% loss of activity compared to wild-type enzyme | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | phenylmethansulfonfluoride has almost no influence on the activity up to 10 mM; PMSF has almost no influence on the activity of LipA up to 10 mM | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.876 | - |
4-nitrophenyl palmitate | pH 7, 50°C | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52800 | - |
x * 52800, calculated from sequence | Archaeoglobus fulgidus |
52800 | - |
x * 52800, SDS-PAGE | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28511 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | converted twice as fast as the corresponding palmitate | Archaeoglobus fulgidus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + palmitate | - |
? | |
butyl acetate + H2O | - |
Archaeoglobus fulgidus | butanol + acetate | - |
? | |
butyl acetate + H2O | the enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols | Archaeoglobus fulgidus | butanol + acetate | - |
? | |
ethyl acetate + H2O | - |
Archaeoglobus fulgidus | ethanol + acetate | - |
? | |
ethyl butyrate + H2O | the enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols | Archaeoglobus fulgidus | ethanol + butanoate | - |
? | |
ethyl propionate + H2O | - |
Archaeoglobus fulgidus | ethanol + propionate | - |
? | |
ethyl propionate + H2O | the enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols | Archaeoglobus fulgidus | ethanol + propionate | - |
? | |
methyl acetate + H2O | - |
Archaeoglobus fulgidus | methanol + acetate | - |
? | |
methyl acetate + H2O | the enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols | Archaeoglobus fulgidus | methanol + acetate | - |
? | |
methyl butyrate + H2O | preferred substrate. The enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols | Archaeoglobus fulgidus | methanol + butanoate | - |
? | |
methyl butyrate + H2O | - |
Archaeoglobus fulgidus | methanol + butyrate | - |
? | |
methyl hexanoate + H2O | - |
Archaeoglobus fulgidus | methanol + hexanoate | - |
? | |
methyl hexanoate + H2O | the enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols | Archaeoglobus fulgidus | methanol + hexanoate | - |
? | |
methyl propionate + H2O | - |
Archaeoglobus fulgidus | methanol + propionate | - |
? | |
methyl propionate + H2O | the enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols | Archaeoglobus fulgidus | methanol + propionate | - |
? | |
additional information | no activity detected with methyl myristate. Tributyrin is hydrolyzed by LipA at a lower rate than short and medium chain fatty acid esters and 4-nitrophenyl acetate is converted twice as fast as the corresponding palmitate | Archaeoglobus fulgidus | ? | - |
? | |
additional information | the enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols. Methyl esters are hydrolyzed faster than the corresponding ethyl esters. Butyrate is the preferred chain length for the carboxylic acid. No activity with esters of hydroxy-substituted fatty acids such as 3-hydroxybutyrate | Archaeoglobus fulgidus | ? | - |
? | |
phenyl acetate + H2O | - |
Archaeoglobus fulgidus | phenol + acetate | - |
? | |
phenyl acetate + H2O | the enzyme preferentially hydrolyzes esters of short-chain fatty acids (C2C4) and especially those of medium chain (C5C6) fatty acids with short chain alcohols | Archaeoglobus fulgidus | phenol + acetate | - |
? | |
tributyrin + H2O | hydrolyzed at a lower rate than short and medium chain fatty acid esters | Archaeoglobus fulgidus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52800, calculated from sequence | Archaeoglobus fulgidus |
? | x * 52800, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
AF1763 | - |
Archaeoglobus fulgidus |
AF1763 | locus name | Archaeoglobus fulgidus |
LipA | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | 40°C: about 40% of maximal activity, 90°C: about 65% of maximal activity | Archaeoglobus fulgidus |
40 | 95 | 40°C: about 40% of maximal activity, 95°C: about 40% of maximal activity | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
about 60% loss of activity after 30 min, about 85% loss of activity after 90 min | Archaeoglobus fulgidus |
40 | - |
about 60% loss of activity after 30 min | Archaeoglobus fulgidus |
60 | - |
about 60% loss of activity after 30 min, about 85% loss of activity after 60 min | Archaeoglobus fulgidus |
60 | - |
about 60% loss of activity after 30 min | Archaeoglobus fulgidus |
80 | - |
30 min, complete loss of activity | Archaeoglobus fulgidus |
80 | - |
complete loss of activity after 30 min | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47.5 | - |
4-nitrophenyl palmitate | pH 7, 50°C | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
at 50°C | Archaeoglobus fulgidus |
10 | 11 | at room temperature | Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | pH 7.0: about 40% of maximal activity, pH 12: about 30% of maximal activity, at room temperature | Archaeoglobus fulgidus |
8 | 12 | pH 8: about 60% of maximal activity, pH 12: about 30% of maximal activity, at room temperature | Archaeoglobus fulgidus |
9 | 12 | pH 9.0: about 70% of maximal activity, pH 12: about 60% of maximal activity, at 50°C | Archaeoglobus fulgidus |
9 | 12 | pH 9: about 70% of maximal activity, pH 12: about 60% of maximal activity, at 50°C | Archaeoglobus fulgidus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54 | - |
4-nitrophenyl palmitate | pH 7, 50°C | Archaeoglobus fulgidus |