Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dimethylformamide | 5% (v/v), about 1.1fold activation | Saccharolobus solfataricus | |
ethanol | 5% (v/v), about 1.2fold activation | Saccharolobus solfataricus | |
methanol | 5-20% (v/v), about 1.2fold activation | Saccharolobus solfataricus |
Application | Comment | Organism |
---|---|---|
synthesis | the enzyme shows chiral resolution activity for (S)-ibuprofen, indicating that the enzyme can be used for the production of commercially important chiral drugs | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | stable at high concentrations of polar organic solvents, such as ethanol and methanol as compared to other mesophilic counterparts | Saccharolobus solfataricus |
Methanol | stable at high concentrations of polar organic solvents, such as ethanol and methanol as compared to other mesophilic counterparts | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus DSM 1617 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.5 | - |
pH 7.0, 75°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-ibuprofen methyl ester + H2O | the enzyme has S-form specificity for ibuprofen | Saccharolobus solfataricus | (S)-ibuprofen + methanol | - |
? | |
(S)-ibuprofen methyl ester + H2O | the enzyme has S-form specificity for ibuprofen | Saccharolobus solfataricus DSM 1617 | (S)-ibuprofen + methanol | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
Saccharolobus solfataricus | 4-methylumbelliferone + acetate | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
Saccharolobus solfataricus DSM 1617 | 4-methylumbelliferone + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Saccharolobus solfataricus DSM 1617 | 4-nitrophenol + butyrate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
110 min, about 10% loss of activity | Saccharolobus solfataricus |
95 | - |
half-life: 45 min | Saccharolobus solfataricus |
100 | - |
half-life: 15 min | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 9.2 | pH 4.8: about 40% of maximal activity, pH 9.2: about 40% of maximal activity | Saccharolobus solfataricus |