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Literature summary for 3.1.1.1 extracted from

  • Shang, Y.S.; Zhang, X.E.; Wang, X.D.; Guo, Y.C.; Zhang, Z.P.; Zhou, Y.F.
    Biochemical characterization and mutational improvement of a thermophilic esterase from Sulfolobus solfataricus P2 (2010), Biotechnol. Lett., 32, 1151-1157.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
F188A activity with 4-nitrophenyl butyrate as substrate is unchanged as compared to the wild-type value Saccharolobus solfataricus
K187A partial loss of activity with 4-nitrophenyl butyrate as substrate Saccharolobus solfataricus
R210A catalytic efficiency (kcat/Km) of the mutant enzyme triples over that of wild-type enzyme Saccharolobus solfataricus
T58A partial loss of activity with 4-nitrophenyl butyrate as substrate Saccharolobus solfataricus
W59A activity with 4-nitrophenyl butyrate as substrate is unchanged as compared to the wild-type value Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
PMSF
-
 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 4-nitrophenyl caprylate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
0.043
-
 4-nitrophenyl caprylate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus
0.63
-
 4-nitrophenyl butyrate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus
0.93
-
 4-nitrophenyl butyrate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58400
-
 x * 58400, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VU1
-
-
Saccharolobus solfataricus P2 Q97VU1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O specific activity is less than 10% compared to the activity with caprylate Saccharolobus solfataricus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O specific activity is less than 10% compared to the activity with caprylate Saccharolobus solfataricus P2 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O specific activity is about 80% compared to the activity with caprylate Saccharolobus solfataricus 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O specific activity is about 80% compared to the activity with caprylate Saccharolobus solfataricus P2 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caprylate + H2O the enzyme shows the highest specific activity with p-nitrophenyl caprylate Saccharolobus solfataricus 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl caprylate + H2O the enzyme shows the highest specific activity with p-nitrophenyl caprylate Saccharolobus solfataricus P2 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl decanoate + H2O specific activity is about 50% compared to the activity with caprylate Saccharolobus solfataricus 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl decanoate + H2O specific activity is about 50% compared to the activity with caprylate Saccharolobus solfataricus P2 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl laurate + H2O specific activity is about 10% compared to the activity with caprylate Saccharolobus solfataricus 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O specific activity is about 10% compared to the activity with caprylate Saccharolobus solfataricus P2 4-nitrophenol + laurate
-
 ?

Subunits

Subunits Comment Organism
? x * 58400, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SsoPEst
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 1 h, about 50% loss of activity Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 4-nitrophenyl caprylate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
4
-
 4-nitrophenyl butyrate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
7.8
-
 4-nitrophenyl caprylate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus
10
-
 4-nitrophenyl butyrate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1
-
 4-nitrophenyl butyrate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
17
-
 4-nitrophenyl butyrate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus
63
-
 4-nitrophenyl caprylate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
192
-
 4-nitrophenyl caprylate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus