Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sarin | for enzyme inhibited by racemic mixtures of bona fide nerve agents, hCE1 spontaneously reactivates in the presence of sarin but not soman or cyclosarin. The addition of 2,3-butanedione monoxime increases the rate of reactivation of CE1 from sarin inhibition by more than 65fold | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in baculovirus-infected Spodoptera frugiperda Sf21 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
carboxylesterase 1 in complex with cyclosarin, sitting drop vapor diffusion method, using 10% (w/v) polyethylene glycol 3350, 0.3 M Li2SO4, 0.1 M citrate, pH 5.5, 0.1 M NaCl, 0.1 M LiCl, and 5% (v/v) glycerol. | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyclosarin | - |
Homo sapiens | |
additional information | 2,3-butanedione monoxime has no inhibitory effect on CE1 | Homo sapiens | |
soman | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Homo sapiens | 4-nitrophenol + butyrate | - |
? | |
additional information | human carboxylesterase 1 stereoselectively binds the nerve agent cyclosarin and exhibits a 1700- and 2900fold preference for the PR enantiomers of analogs of soman and cyclosarin, respectively | Homo sapiens | ? | - |
? | |
sarin + H2O | CE1 exhibits a 5fold preference for the PS isomer of a sarin analog | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carboxylesterase 1 | - |
Homo sapiens |
CE1 | - |
Homo sapiens |