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Literature summary for 3.1.1.1 extracted from

  • Aurilia, V.; Parracino, A.; DAuria, S.
    Microbial carbohydrate esterases in cold adapted environments (2008), Gene, 410, 234-240.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudoalteromonas haloplanktis

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme activity is not affected by the presence of 5.0 mM phenylmethylsulfonyl fluoride Pseudoalteromonas haloplanktis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates the enzyme activity Pseudoalteromonas haloplanktis
Mg2+ stimulates the enzyme activity Pseudoalteromonas haloplanktis
Mn2+ stimulates the enzyme activity Pseudoalteromonas haloplanktis

Organic Solvent Stability

Organic Solvent Comment Organism
SDS the enzyme activity is not affected by the presence of SDS at up 0.1% Pseudoalteromonas haloplanktis

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas haloplanktis
-
-
-
Pseudoalteromonas haloplanktis TAC 125
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl p-trimethylammoniocinnamate chloride + H2O
-
Pseudoalteromonas haloplanktis 4-methylumbelliferol + p-trimethylammoniocinnamate chloride
-
?
4-methylumbelliferyl p-trimethylammoniocinnamate chloride + H2O
-
Pseudoalteromonas haloplanktis TAC 125 4-methylumbelliferol + p-trimethylammoniocinnamate chloride
-
?
4-nitrophenyl acetate + H2O
-
Pseudoalteromonas haloplanktis 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + acetate
-
?
alpha-naphthyl acetate + H2O
-
Pseudoalteromonas haloplanktis alpha-naphthol + acetate
-
?
alpha-naphthyl acetate + H2O
-
Pseudoalteromonas haloplanktis TAC 125 alpha-naphthol + acetate
-
?
beta-naphthyl acetate + H2O
-
Pseudoalteromonas haloplanktis beta-naphthol + acetate
-
?
beta-naphthyl acetate + H2O
-
Pseudoalteromonas haloplanktis TAC 125 beta-naphthol + acetate
-
?
additional information the enzyme does not show any activity towards p-nitrophenyl esanoate and p-nitrophenyl dodecanoate Pseudoalteromonas haloplanktis ?
-
?
additional information the enzyme does not show any activity towards p-nitrophenyl esanoate and p-nitrophenyl dodecanoate Pseudoalteromonas haloplanktis TAC 125 ?
-
?

Synonyms

Synonyms Comment Organism
triacylglycerol hydrolase
-
Pseudoalteromonas haloplanktis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
Pseudoalteromonas haloplanktis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 20 the enzyme, incubated at 20°C, shows a partial de-activation after 12 h, the enzyme incubated at 4°C displays a constant activity over a 3 days period Pseudoalteromonas haloplanktis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudoalteromonas haloplanktis