Cloned (Comment) | Organism |
---|---|
expression as glutathione S-transferase fusion protein in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D450A | complete loss of activity | Mycobacterium tuberculosis |
H477A | complete loss of activity | Mycobacterium tuberculosis |
additional information | enzyme deletion results in reduced colony-forming unit loads in lungs and spleen and less lung pathology in BALB/mouse aerosol infection. Intravenous infection of mice with mutant cells results in significantly delayed time to death | Mycobacterium tuberculosis |
S215A | complete loss of activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dichlorvos | - |
Mycobacterium tuberculosis | |
paraoxon | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.738 | - |
4-methylumbelliferyl butanoate | pH 7.0, 39°C | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | associated to | Mycobacterium tuberculosis | 5618 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
isoform MT2282 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | lipoprotein associated with membrane, sequence analysis | Mycobacterium tuberculosis |
proteolytic modification | sequence contains a N-terminal signal peptide | Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
recombinant protein | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl butanoate + H2O | enzyme activity in decreasing order: 4-methylumbelliferyl butanoate, 4-methylumbelliferyl heptanoate, 4-methylumbelliferyl oleate | Mycobacterium tuberculosis | 4-methylumbelliferol + butanoate | - |
? | |
4-methylumbelliferyl heptanoate + H2O | enzyme activity in decreasing order: 4-methylumbelliferyl butanoate, 4-methylumbelliferyl heptanoate, 4-methylumbelliferyl oleate | Mycobacterium tuberculosis | 4-methylumbelliferol + heptanoate | - |
? | |
4-methylumbelliferyl oleate + H2O | enzyme activity in decreasing order: 4-methylumbelliferyl butanoate, 4-methylumbelliferyl heptanoate, 4-methylumbelliferyl oleate | Mycobacterium tuberculosis | 4-methylumbelliferol + oleate | - |
? | |
alpha-naphthyl acetate + H2O | enzyme activity increases from acetate to propionate to butanoate substrate | Mycobacterium tuberculosis | alpha-naphthol + acetate | - |
? | |
alpha-naphthyl butanoate + H2O | enzyme activity increases from acetate to propionate to butanoate substrate | Mycobacterium tuberculosis | alpha-naphthol + butanoate | - |
? | |
alpha-naphthyl propionate + H2O | enzyme activity increases from acetate to propionate to butanoate substrate | Mycobacterium tuberculosis | alpha-naphthol + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 54000, calculated | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
MT2282 | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity is 10% lower than at 39°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mycobacterium tuberculosis |