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Literature summary for 3.1.1.1 extracted from

  • Boettcher, D.; Bruesehaber, E.; Doderer, K.; Bornscheuer, U.T.
    Functional expression of the gamma-isoenzyme of pig liver carboxyl esterase in Escherichia coli (2007), Appl. Microbiol. Biotechnol., 73, 1282-1289.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged expression in Escherichia coli, with coexpresssion of chaperones GroEL and GroES. Expression in Pichia pastoris Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information ratio of vmax to Km value is 0.0108 per min for enzyme purified from Escherichia coli, 0.109 per min for enzyme expressed in Pichia pastoris Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
3 * 63000, SDS-PAGE of glycosylated protein, 3 * 58000, SDS-PAGE of non-glycosylated protein. 3 * 61500, calculated Sus scrofa
61500
-
3 * 63000, SDS-PAGE of glycosylated protein, 3 * 58000, SDS-PAGE of non-glycosylated protein. 3 * 61500, calculated Sus scrofa
63000
-
3 * 63000, SDS-PAGE of glycosylated protein, 3 * 58000, SDS-PAGE of non-glycosylated protein. 3 * 61500, calculated Sus scrofa
175000
-
gel filtration Sus scrofa
175000
-
Ferguson analysis Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
isoform gamma-rPLE
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 63000 Da, SDS-PAGE of glycosylated protein, 58000 Da, SDS-PAGE of non-glycosylated protein Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
92
-
pH 7.5 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl caprylate + H2O
-
Sus scrofa ethanol + caprylate
-
?
methyl acetate poor substrate Sus scrofa methanol + acetate
-
?
p-nitrophenyl acetate + H2O
-
Sus scrofa p-nitrophenol + acetate
-
?
tributyrin + H2O
-
Sus scrofa butanoate + glycerol
-
?

Subunits

Subunits Comment Organism
trimer 3 * 63000, SDS-PAGE of glycosylated protein, 3 * 58000, SDS-PAGE of non-glycosylated protein. 3 * 61500, calculated Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 70 more than 90% of maximum activity Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
and higher pH optimum at 8.0 Sus scrofa
8
-
and lower pH optimum at 6.0 Sus scrofa