Cloned (Comment) | Organism |
---|---|
His-tagged expression in Escherichia coli, with coexpresssion of chaperones GroEL and GroES. Expression in Pichia pastoris | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ratio of vmax to Km value is 0.0108 per min for enzyme purified from Escherichia coli, 0.109 per min for enzyme expressed in Pichia pastoris | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
3 * 63000, SDS-PAGE of glycosylated protein, 3 * 58000, SDS-PAGE of non-glycosylated protein. 3 * 61500, calculated | Sus scrofa |
61500 | - |
3 * 63000, SDS-PAGE of glycosylated protein, 3 * 58000, SDS-PAGE of non-glycosylated protein. 3 * 61500, calculated | Sus scrofa |
63000 | - |
3 * 63000, SDS-PAGE of glycosylated protein, 3 * 58000, SDS-PAGE of non-glycosylated protein. 3 * 61500, calculated | Sus scrofa |
175000 | - |
gel filtration | Sus scrofa |
175000 | - |
Ferguson analysis | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
isoform gamma-rPLE | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 63000 Da, SDS-PAGE of glycosylated protein, 58000 Da, SDS-PAGE of non-glycosylated protein | Sus scrofa |
Purification (Comment) | Organism |
---|---|
recombinant protein | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
92 | - |
pH 7.5 | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl caprylate + H2O | - |
Sus scrofa | ethanol + caprylate | - |
? | |
methyl acetate | poor substrate | Sus scrofa | methanol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Sus scrofa | p-nitrophenol + acetate | - |
? | |
tributyrin + H2O | - |
Sus scrofa | butanoate + glycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 63000, SDS-PAGE of glycosylated protein, 3 * 58000, SDS-PAGE of non-glycosylated protein. 3 * 61500, calculated | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 70 | more than 90% of maximum activity | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
and higher pH optimum at 8.0 | Sus scrofa |
8 | - |
and lower pH optimum at 6.0 | Sus scrofa |