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Literature summary for 3.1.1.1 extracted from

  • Elend, C.; Schmeisser, C.; Leggewie, C.; Babiak, P.; Carballeira, J.D.; Steele, H.L.; Reymond, J.; Jaeger, K.; Streit, W.R.
    Isolation and biochemical characterization of two novel metagenome-derived esterases (2006), Appl. Environ. Microbiol., 72, 3637-3645.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
acetone 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity unidentified
acetonitrile 15% v/v, 110% of initial activity, 30% v/v, 87% of initial activity unidentified
dimethylformamide 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity unidentified
EDTA slight activation unidentified soil organism
Isopropanol 15% v/v, 155% of initial activity, 30% v/v, 117% of initial activity unidentified
methanol 15% v/v, 111% of initial activity, 30% v/v, 130% of initial activity unidentified

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli unidentified
expression in Escherichia coli unidentified soil organism

Protein Variants

Protein Variants Comment Organism
S61A no catalytic activity at 20°C or 45°C unidentified
S65A no catalytic activity at 20°C or 45°C unidentified soil organism

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
unidentified
Cu2+
-
unidentified
Cu2+ strong unidentified soil organism
EDTA
-
unidentified
Mg2+
-
unidentified
Rb+
-
unidentified
sodium dodecylsulfate 0.1%, no effect during short-term incubation, complete inhibition after 60 min unidentified
sodium dodecylsulfate 0.1%, no effect during short-term incubation, complete inhibition after 60 min unidentified soil organism
Zn2+
-
unidentified
Zn2+
-
unidentified soil organism

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation unidentified soil organism
Co2+ 33% activation unidentified
Mg2+ slight activation unidentified soil organism

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein unidentified
42100
-
x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein unidentified soil organism
43300
-
x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein unidentified
44000
-
x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein unidentified soil organism
260000
-
gel filtration unidentified
260000
-
gel filtration unidentified soil organism

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity unidentified
Acetone 15% v/v, 14% of initial activity unidentified soil organism
acetonitrile 15% v/v, 0% of initial activity unidentified soil organism
acetonitrile 15% v/v, 110% of initial activity, 30% v/v, 87% of initial activity unidentified
dimethyl sulfoxide up to 15% v/V, enzyme retains full activity unidentified soil organism
dimethyl sulfoxide up to 30% v/V, enzyme retains full activity unidentified
dimethylformamide 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity unidentified
dimethylformamide 15% v/v, 34% of initial activity unidentified soil organism
isopropanol 15% v/v, 155% of initial activity, 30% v/v, 117% of initial activity unidentified
isopropanol 15% v/v, 75% of initial activity unidentified soil organism
Methanol 15% v/v, 111% of initial activity, 30% v/v, 130% of initial activity unidentified
Methanol 15% v/v, 90% of initial activity unidentified soil organism

Organism

Organism UniProt Comment Textmining
unidentified
-
enzyme EstA3, isolated from drinking water genome
-
unidentified soil organism
-
enzyme EstCE1, isolated from soil metagenome
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme unidentified
recombinant enzyme unidentified soil organism

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
substrate p-nitrophenyl octanoate, 45°C, pH 7.0 unidentified soil organism
3.6
-
substrate p-nitrophenyl octanoate, 45°C, pH 7.0 unidentified
7.4
-
substrate p-nitrophenyl caproate, 45°C, pH 7.0 unidentified soil organism
13.3
-
substrate vinyl acetate, 45°C, pH 7.0 unidentified soil organism
13.3
-
substrate vinyl propionate, 45°C, pH 7.0 unidentified soil organism
16
-
substrate vinyl butanoate, 45°C, pH 7.0 unidentified soil organism
22.9
-
substrate p-nitrophenyl valerate, 45°C, pH 7.0 unidentified soil organism
26.7
-
substrate triacetin, 45°C, pH 7.0 unidentified soil organism
30.9
-
substrate p-nitrophenyl decanoate, 45°C, pH 7.0 unidentified soil organism
31.1
-
substrate p-nitrophenyl butanoate, 45°C, pH 7.0 unidentified soil organism
33.3
-
substrate tripropionin, 45°C, pH 7.0 unidentified soil organism
54.7
-
substrate tributyrin, 45°C, pH 7.0 unidentified soil organism
107.5
-
substrate vinyl acetate, 45°C, pH 7.0 unidentified
147.5
-
substrate triacetin, 45°C, pH 7.0 unidentified
167.5
-
substrate tributyrin, 45°C, pH 7.0 unidentified
380
-
substrate vinyl propionate, 45°C, pH 7.0 unidentified
387.5
-
substrate vinyl butanoate, 45°C, pH 7.0 unidentified
387.5
-
substrate vinyl caproate, 45°C, pH 7.0 unidentified
387.5
-
substrate vinyl caprylate, 45°C, pH 7.0 unidentified
425.7
-
substrate p-nitrophenyl caproate, 45°C, pH 7.0 unidentified
425.7
-
substrate p-nitrophenyl valerate, 45°C, pH 7.0 unidentified
513.6
-
substrate p-nitrophenyl butanoate, 45°C, pH 7.0 unidentified

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-menthylacetate + H2O enzyme is highly enantioselective for (+)-menthylacetate unidentified soil organism (+)-menthol + acetate
-
?
7-[4-octylcarboxy-(3-hydroxy-3-methyl)-butyloxy]-coumarin + H2O
-
unidentified ?
-
?
additional information enzyme does not show beta-lactamase activity unidentified ?
-
?
additional information enzyme does not show beta-lactamase activity unidentified soil organism ?
-
?
p-nitrophenyl butanoate + H2O
-
unidentified p-nitrophenol + butanoate
-
?
p-nitrophenyl butanoate + H2O
-
unidentified soil organism p-nitrophenol + butanoate
-
?
p-nitrophenyl caproate
-
unidentified p-nitrophenol + caproate
-
?
p-nitrophenyl caproate
-
unidentified soil organism p-nitrophenol + caproate
-
?
p-nitrophenyl decanoate
-
unidentified soil organism p-nitrophenol + octanoate
-
?
p-nitrophenyl octanoate
-
unidentified p-nitrophenol + octanoate
-
?
p-nitrophenyl octanoate
-
unidentified soil organism p-nitrophenol + octanoate
-
?
p-nitrophenyl palmitate + H2O
-
unidentified p-nitrophenol + palmitate
-
?
p-nitrophenyl valerate + H2O
-
unidentified p-nitrophenol + valeric acid
-
?
p-nitrophenyl valerate + H2O
-
unidentified soil organism p-nitrophenol + valeric acid
-
?
triacetin + H2O
-
unidentified acetic acid + glycerol
-
?
triacetin + H2O
-
unidentified soil organism acetic acid + glycerol
-
?
tributyrin + H2O
-
unidentified butanoate + glycerol
-
?
tributyrin + H2O
-
unidentified soil organism butanoate + glycerol
-
?
tripropionin + H2O
-
unidentified propanoic acid + glycerol
-
?
tripropionin + H2O
-
unidentified soil organism propanoic acid + glycerol
-
?
vinyl acetate + H2O
-
unidentified vinyl alcohol + acetate
-
?
vinyl acetate + H2O
-
unidentified soil organism vinyl alcohol + acetate
-
?
vinyl butanoate + H2O
-
unidentified vinyl alcohol + butanoate
-
?
vinyl butanoate + H2O
-
unidentified soil organism vinyl alcohol + butanoate
-
?
vinyl caproate + H2O
-
unidentified vinyl alcohol + caproate
-
?
vinyl caprylate + H2O
-
unidentified vinyl alcohol + caprylate
-
?
vinyl propionate + H2O
-
unidentified vinyl alcohol + propionate
-
?
vinyl propionate + H2O
-
unidentified soil organism vinyl alcohol + propionate
-
?

Subunits

Subunits Comment Organism
multimer x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein unidentified
multimer x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein unidentified soil organism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
47
-
-
unidentified soil organism
50
-
-
unidentified

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
36 51 at least 50% of maximum activity within this range unidentified soil organism
38 56 at least 50% of maximum activity within this range unidentified

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
60 min, stable unidentified
40
-
60 min, stable unidentified soil organism
45
-
rapid inactivation when incubated for more than 60 min unidentified
45
-
rapid inactivation when incubated for more than 60 min unidentified soil organism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
unidentified
10
-
-
unidentified soil organism

pH Range

pH Minimum pH Maximum Comment Organism
4
-
no activity below unidentified
5
-
no activity below unidentified soil organism

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 little loss of catalytic activity after incubation for up to 48 h unidentified
1 2 little loss of catalytic activity after incubation for up to 48 h unidentified soil organism