Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity | unidentified | |
acetonitrile | 15% v/v, 110% of initial activity, 30% v/v, 87% of initial activity | unidentified | |
dimethylformamide | 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity | unidentified | |
EDTA | slight activation | unidentified soil organism | |
Isopropanol | 15% v/v, 155% of initial activity, 30% v/v, 117% of initial activity | unidentified | |
methanol | 15% v/v, 111% of initial activity, 30% v/v, 130% of initial activity | unidentified |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | unidentified |
expression in Escherichia coli | unidentified soil organism |
Protein Variants | Comment | Organism |
---|---|---|
S61A | no catalytic activity at 20°C or 45°C | unidentified |
S65A | no catalytic activity at 20°C or 45°C | unidentified soil organism |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
unidentified | |
Cu2+ | - |
unidentified | |
Cu2+ | strong | unidentified soil organism | |
EDTA | - |
unidentified | |
Mg2+ | - |
unidentified | |
Rb+ | - |
unidentified | |
sodium dodecylsulfate | 0.1%, no effect during short-term incubation, complete inhibition after 60 min | unidentified | |
sodium dodecylsulfate | 0.1%, no effect during short-term incubation, complete inhibition after 60 min | unidentified soil organism | |
Zn2+ | - |
unidentified | |
Zn2+ | - |
unidentified soil organism |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation | unidentified soil organism | |
Co2+ | 33% activation | unidentified | |
Mg2+ | slight activation | unidentified soil organism |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein | unidentified |
42100 | - |
x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein | unidentified soil organism |
43300 | - |
x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein | unidentified |
44000 | - |
x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein | unidentified soil organism |
260000 | - |
gel filtration | unidentified |
260000 | - |
gel filtration | unidentified soil organism |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity | unidentified |
Acetone | 15% v/v, 14% of initial activity | unidentified soil organism |
acetonitrile | 15% v/v, 0% of initial activity | unidentified soil organism |
acetonitrile | 15% v/v, 110% of initial activity, 30% v/v, 87% of initial activity | unidentified |
dimethyl sulfoxide | up to 15% v/V, enzyme retains full activity | unidentified soil organism |
dimethyl sulfoxide | up to 30% v/V, enzyme retains full activity | unidentified |
dimethylformamide | 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity | unidentified |
dimethylformamide | 15% v/v, 34% of initial activity | unidentified soil organism |
isopropanol | 15% v/v, 155% of initial activity, 30% v/v, 117% of initial activity | unidentified |
isopropanol | 15% v/v, 75% of initial activity | unidentified soil organism |
Methanol | 15% v/v, 111% of initial activity, 30% v/v, 130% of initial activity | unidentified |
Methanol | 15% v/v, 90% of initial activity | unidentified soil organism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
unidentified | - |
enzyme EstA3, isolated from drinking water genome | - |
unidentified soil organism | - |
enzyme EstCE1, isolated from soil metagenome | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | unidentified |
recombinant enzyme | unidentified soil organism |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
substrate p-nitrophenyl octanoate, 45°C, pH 7.0 | unidentified soil organism |
3.6 | - |
substrate p-nitrophenyl octanoate, 45°C, pH 7.0 | unidentified |
7.4 | - |
substrate p-nitrophenyl caproate, 45°C, pH 7.0 | unidentified soil organism |
13.3 | - |
substrate vinyl acetate, 45°C, pH 7.0 | unidentified soil organism |
13.3 | - |
substrate vinyl propionate, 45°C, pH 7.0 | unidentified soil organism |
16 | - |
substrate vinyl butanoate, 45°C, pH 7.0 | unidentified soil organism |
22.9 | - |
substrate p-nitrophenyl valerate, 45°C, pH 7.0 | unidentified soil organism |
26.7 | - |
substrate triacetin, 45°C, pH 7.0 | unidentified soil organism |
30.9 | - |
substrate p-nitrophenyl decanoate, 45°C, pH 7.0 | unidentified soil organism |
31.1 | - |
substrate p-nitrophenyl butanoate, 45°C, pH 7.0 | unidentified soil organism |
33.3 | - |
substrate tripropionin, 45°C, pH 7.0 | unidentified soil organism |
54.7 | - |
substrate tributyrin, 45°C, pH 7.0 | unidentified soil organism |
107.5 | - |
substrate vinyl acetate, 45°C, pH 7.0 | unidentified |
147.5 | - |
substrate triacetin, 45°C, pH 7.0 | unidentified |
167.5 | - |
substrate tributyrin, 45°C, pH 7.0 | unidentified |
380 | - |
substrate vinyl propionate, 45°C, pH 7.0 | unidentified |
387.5 | - |
substrate vinyl butanoate, 45°C, pH 7.0 | unidentified |
387.5 | - |
substrate vinyl caproate, 45°C, pH 7.0 | unidentified |
387.5 | - |
substrate vinyl caprylate, 45°C, pH 7.0 | unidentified |
425.7 | - |
substrate p-nitrophenyl caproate, 45°C, pH 7.0 | unidentified |
425.7 | - |
substrate p-nitrophenyl valerate, 45°C, pH 7.0 | unidentified |
513.6 | - |
substrate p-nitrophenyl butanoate, 45°C, pH 7.0 | unidentified |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-menthylacetate + H2O | enzyme is highly enantioselective for (+)-menthylacetate | unidentified soil organism | (+)-menthol + acetate | - |
? | |
7-[4-octylcarboxy-(3-hydroxy-3-methyl)-butyloxy]-coumarin + H2O | - |
unidentified | ? | - |
? | |
additional information | enzyme does not show beta-lactamase activity | unidentified | ? | - |
? | |
additional information | enzyme does not show beta-lactamase activity | unidentified soil organism | ? | - |
? | |
p-nitrophenyl butanoate + H2O | - |
unidentified | p-nitrophenol + butanoate | - |
? | |
p-nitrophenyl butanoate + H2O | - |
unidentified soil organism | p-nitrophenol + butanoate | - |
? | |
p-nitrophenyl caproate | - |
unidentified | p-nitrophenol + caproate | - |
? | |
p-nitrophenyl caproate | - |
unidentified soil organism | p-nitrophenol + caproate | - |
? | |
p-nitrophenyl decanoate | - |
unidentified soil organism | p-nitrophenol + octanoate | - |
? | |
p-nitrophenyl octanoate | - |
unidentified | p-nitrophenol + octanoate | - |
? | |
p-nitrophenyl octanoate | - |
unidentified soil organism | p-nitrophenol + octanoate | - |
? | |
p-nitrophenyl palmitate + H2O | - |
unidentified | p-nitrophenol + palmitate | - |
? | |
p-nitrophenyl valerate + H2O | - |
unidentified | p-nitrophenol + valeric acid | - |
? | |
p-nitrophenyl valerate + H2O | - |
unidentified soil organism | p-nitrophenol + valeric acid | - |
? | |
triacetin + H2O | - |
unidentified | acetic acid + glycerol | - |
? | |
triacetin + H2O | - |
unidentified soil organism | acetic acid + glycerol | - |
? | |
tributyrin + H2O | - |
unidentified | butanoate + glycerol | - |
? | |
tributyrin + H2O | - |
unidentified soil organism | butanoate + glycerol | - |
? | |
tripropionin + H2O | - |
unidentified | propanoic acid + glycerol | - |
? | |
tripropionin + H2O | - |
unidentified soil organism | propanoic acid + glycerol | - |
? | |
vinyl acetate + H2O | - |
unidentified | vinyl alcohol + acetate | - |
? | |
vinyl acetate + H2O | - |
unidentified soil organism | vinyl alcohol + acetate | - |
? | |
vinyl butanoate + H2O | - |
unidentified | vinyl alcohol + butanoate | - |
? | |
vinyl butanoate + H2O | - |
unidentified soil organism | vinyl alcohol + butanoate | - |
? | |
vinyl caproate + H2O | - |
unidentified | vinyl alcohol + caproate | - |
? | |
vinyl caprylate + H2O | - |
unidentified | vinyl alcohol + caprylate | - |
? | |
vinyl propionate + H2O | - |
unidentified | vinyl alcohol + propionate | - |
? | |
vinyl propionate + H2O | - |
unidentified soil organism | vinyl alcohol + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein | unidentified |
multimer | x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein | unidentified soil organism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
- |
unidentified soil organism |
50 | - |
- |
unidentified |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | 51 | at least 50% of maximum activity within this range | unidentified soil organism |
38 | 56 | at least 50% of maximum activity within this range | unidentified |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
60 min, stable | unidentified |
40 | - |
60 min, stable | unidentified soil organism |
45 | - |
rapid inactivation when incubated for more than 60 min | unidentified |
45 | - |
rapid inactivation when incubated for more than 60 min | unidentified soil organism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
unidentified |
10 | - |
- |
unidentified soil organism |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | - |
no activity below | unidentified |
5 | - |
no activity below | unidentified soil organism |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | little loss of catalytic activity after incubation for up to 48 h | unidentified |
1 | 2 | little loss of catalytic activity after incubation for up to 48 h | unidentified soil organism |