Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Bacillus subtilis |
34000 | - |
x * 34000, SDS-PAGE of both isoforms CesA and CesB | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
isoform CesA, CesB | - |
Bacillus subtilis | - |
isoform NP | - |
Bacillus subtilis 168 | - |
isoform CesA, CesB | - |
Bacillus subtilis Thai I-8 | - |
isoform NP | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
isoform CesB, substrate beta-naphthylacetate, pH 7.5, 32°C | Bacillus subtilis |
0.03 | - |
isoform Cesb, substrate (R)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
0.04 | - |
isoform CesA, substrate (R)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
0.04 | - |
substrate (R)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
0.08 | - |
isoform CesA, substrate beta-naphthylacetate, pH 7.5, 32°C | Bacillus subtilis |
0.1 | - |
substrate beta-naphthylacetate, pH 7.5, 32°C | Bacillus subtilis |
0.17 | - |
isoform CesB, substrate (S)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
0.55 | - |
isoform CesB, substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
4.23 | - |
isoform CesA, substrate (S)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
4.6 | - |
substrate (S)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
20.9 | - |
substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
25.4 | - |
isoform CesA, substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis | (+)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
(+)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis 168 | (+)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
(+)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis Thai I-8 | (+)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
(+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | - |
Bacillus subtilis | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
(+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | no substrate for isoform CesB | Bacillus subtilis | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
(+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | - |
Bacillus subtilis 168 | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
(+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | no substrate for isoform CesB | Bacillus subtilis 168 | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
(+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | - |
Bacillus subtilis Thai I-8 | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
(+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | no substrate for isoform CesB | Bacillus subtilis Thai I-8 | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
(-)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis | (-)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
(-)-1,2-O-isopropylidenglycerol caprylate + H2O | no substrate for isoform CesB | Bacillus subtilis | (-)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
(-)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis 168 | (-)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
(-)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis Thai I-8 | (-)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
(R)-naproxen methyl ester + H2O | - |
Bacillus subtilis | (R)-naproxen + methanol | - |
? | |
(S)-naproxen methyl ester + H2O | - |
Bacillus subtilis | (S)-naproxen + methanol | - |
? | |
2-naphthyl acetate + H2O | - |
Bacillus subtilis | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Bacillus subtilis 168 | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Bacillus subtilis Thai I-8 | 2-naphthol + acetate | - |
? | |
Rac-ibuprofen methyl ester + H2O | - |
Bacillus subtilis | Rac-ibuprofen + methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Bacillus subtilis |
? | x * 34000, SDS-PAGE of both isoforms CesA and CesB | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bacillus subtilis |
30 | - |
isoform CesA | Bacillus subtilis |
40 | - |
isoform CesB | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
no enzymic activity above | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus subtilis |
7.5 | - |
both CesA and CesB | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
inactive below | Bacillus subtilis |