Protein Variants | Comment | Organism |
---|---|---|
G244P | significant increase in thermostability at 40°C, shift of acyl-chain preference towards higher preference for short-chain fqtty acid esters | Psychrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
4-nitrophenyl octanoate | wild-type, pH 8.0, 25°C | Psychrobacter sp. | |
0.07 | - |
4-nitrophenyl hexanoate | wild-type, pH 8.0, 25°C | Psychrobacter sp. | |
0.19 | - |
4-nitrophenyl valerate | wild-type, pH 8.0, 25°C | Psychrobacter sp. | |
0.25 | - |
4-nitrophenyl hexanoate | mutant G244P, pH 8.0, 25°C | Psychrobacter sp. | |
0.28 | - |
4-nitrophenyl butanoate | wild-type, pH 8.0, 25°C | Psychrobacter sp. | |
0.33 | - |
4-nitrophenyl valerate | mutant G244P, pH 8.0, 25°C | Psychrobacter sp. | |
0.42 | - |
4-nitrophenyl butanoate | mutant G244P, pH 8.0, 25°C | Psychrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychrobacter sp. | - |
- |
- |
Psychrobacter sp. Ant300 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butanoate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Psychrobacter sp. Ant300 | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Psychrobacter sp. Ant300 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Psychrobacter sp. Ant300 | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + valerate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Psychrobacter sp. Ant300 | 4-nitrophenol + valerate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7.9, wild-type, half-life 16 min, mutant G244P, half-life 11.6 h | Psychrobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
4-nitrophenyl hexanoate | mutant G244P, pH 8.0, 25°C | Psychrobacter sp. | |
24 | - |
4-nitrophenyl octanoate | wild-type, pH 8.0, 25°C | Psychrobacter sp. | |
58 | - |
4-nitrophenyl hexanoate | wild-type, pH 8.0, 25°C | Psychrobacter sp. | |
74 | - |
4-nitrophenyl valerate | mutant G244P, pH 8.0, 25°C | Psychrobacter sp. | |
110 | - |
4-nitrophenyl butanoate | wild-type, pH 8.0, 25°C | Psychrobacter sp. | |
110 | - |
4-nitrophenyl valerate | wild-type, pH 8.0, 25°C | Psychrobacter sp. | |
110 | - |
4-nitrophenyl butanoate | mutant G244P, pH 8.0, 25°C | Psychrobacter sp. |