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Literature summary for 3.1.1.1 extracted from
- Purification and characterization of PrbA, a new esterase from Enterobacter cloacae hydrolyzing the esters of 4-hydroxybenzoic acid (parabens) (2003), J. Biol. Chem., 278, 12779-12785.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-chloro-3-tosylamido-4-phenyl-2-butanone | - |
Enterobacter cloacae |  |
| 1-chloro-3-tosylamido-7-amino-2-heptanone | - |
Enterobacter cloacae | |
| diethyl dicarbonate | irreversible inhibition | Enterobacter cloacae | |
| diisopropyl fluorophosphate | irreversible inhibition | Enterobacter cloacae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
butyl paraben | pH 6.8, 30°C | Enterobacter cloacae | |
| 0.6 | - |
propyl paraben | pH 6.8, 30°C | Enterobacter cloacae | |
| 0.88 | - |
methyl paraben | pH 6.8, 30°C | Enterobacter cloacae | |
| 3.7 | - |
4-nitrophenyl acetate | pH 6.8, 30°C | Enterobacter cloacae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54620 | - |
mass spectrometry | Enterobacter cloacae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | - |
gene name prbA | - |
| Enterobacter cloacae EM | - |
gene name prbA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 60fold | Enterobacter cloacae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00023 | - |
purified enzyme, substrate 4-nitrophenyl acetate | Enterobacter cloacae |
| 0.0009 | - |
purified enzyme, substrate butyl paraben | Enterobacter cloacae |
| 0.0019 | - |
purified enzyme, substrate propyl paraben | Enterobacter cloacae |
| 0.0025 | - |
purified enzyme, substrate methyl paraben | Enterobacter cloacae |
| 0.0027 | - |
purified enzyme, substrate ethyl paraben | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Enterobacter cloacae | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Enterobacter cloacae EM | 4-nitrophenol + acetate | - |
? | |
| butyl paraben + H2O | - |
Enterobacter cloacae | butanol + 4-hydroxybenzoate | - |
? | |
| ethyl 3,4-dihydroxybenzoate + H2O | low activity | Enterobacter cloacae | ethanol + 3,4-dihydroxybenzoate | - |
? | |
| ethyl paraben + H2O | best substrate | Enterobacter cloacae | ethanol + 4-hydroxybenzoate | - |
? | |
| ethyl paraben + H2O | best substrate | Enterobacter cloacae EM | ethanol + 4-hydroxybenzoate | - |
? | |
| methyl 2-aminobenzoate + H2O | - |
Enterobacter cloacae | methanol + 2-aminobenzoate | - |
? | |
| methyl 2-hydroxybenzoate + H2O | very low activity | Enterobacter cloacae | methanol + 2-hydroxybenzoate | - |
? | |
| methyl 3,5-dihydroxybenzoate + H2O | low activity | Enterobacter cloacae | methanol + 3-hydroxybenzoate | - |
? | |
| methyl 3-hydroxybenzoate + H2O | - |
Enterobacter cloacae | methanol + 3-hydroxybenzoate | - |
? | |
| methyl 4-aminobenzoate + H2O | - |
Enterobacter cloacae | methanol + 4-aminobenzoate | - |
? | |
| methyl benzoate + H2O | low activity | Enterobacter cloacae | methanol + benzoate | - |
? | |
| methyl paraben + H2O | - |
Enterobacter cloacae | methanol + 4-hydroxybenzoate | - |
? | |
| methyl paraben + H2O | - |
Enterobacter cloacae EM | methanol + 4-hydroxybenzoate | - |
? | |
| methyl vanillate + H2O | - |
Enterobacter cloacae | methanol + vanillate | - |
? | |
| additional information | the enzyme also performs transesterification reactions in competition to the hydrolysis, overview | Enterobacter cloacae | ? | - |
? | |
| additional information | the enzyme also performs transesterification reactions in competition to the hydrolysis, overview | Enterobacter cloacae EM | ? | - |
? | |
| propyl paraben + H2O | - |
Enterobacter cloacae | propanol + 4-hydroxybenzoate | - |
? | |
| propyl paraben + H2O | - |
Enterobacter cloacae EM | propanol + 4-hydroxybenzoate | - |
? | |
| trans-methyl cinnamate + H2O | very low activity | Enterobacter cloacae | methanol + cinnamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PrbA | - |
Enterobacter cloacae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 31 | - |
- |
Enterobacter cloacae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | more than 50% of maximal activity at 15°C and 50°C | Enterobacter cloacae |
| 29 | 35 | 90% of maximal activity at 29°C and 35°C | Enterobacter cloacae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Enterobacter cloacae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 70-80% of maximal activity at pH 4.0 and pH 8.0 | Enterobacter cloacae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
1-chloro-3-tosylamido-7-amino-2-heptanone | pH 6.8, 30°C | Enterobacter cloacae | |
| 0.29 | - |
1-chloro-3-tosylamido-4-phenyl-2-butanone | pH 6.8, 30°C | Enterobacter cloacae | |
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