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Literature summary for 3.1.1.1 extracted from

  • Uchida, H.; Kurakata, Y.; Sawamura, H.; Inamura, N.; Kotani, T.; Uwajima, T.
    Purification and properties of an esterase from Aspergillus nomius HS-1 degrading ethylene glycol dibenzoate (2003), FEMS Microbiol. Lett., 223, 123-127.
    View publication on PubMed

Application

Application Comment Organism
biotechnology enzyme is an important biocatalyst for the preparation of a wide range of selectively protected and enantiomerically pure compounds Aspergillus nomiae

Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by EDTA, Ca2+, Mg2+, Co2+, Fe3+, Zn2+ Aspergillus nomiae
PMSF complete inhibition Aspergillus nomiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Aspergillus nomiae
0.034
-
4-nitrophenyl valerate pH 7.0, 37°C Aspergillus nomiae
0.06
-
4-nitrophenyl butyrate pH 7.0, 37°C Aspergillus nomiae
0.075
-
n-butyl benzoate pH 7.0, 37°C Aspergillus nomiae
0.11
-
ethyl benzoate pH 7.0, 37°C Aspergillus nomiae
0.33
-
4-nitrophenyl caproate pH 7.0, 37°C Aspergillus nomiae
0.35
-
4-nitrophenyl propionate pH 7.0, 37°C Aspergillus nomiae
0.39
-
ethyl 4-hydroxybenzoate pH 7.0, 37°C Aspergillus nomiae
0.66
-
ethylene glycol dibenzoate pH 7.0, 37°C Aspergillus nomiae
1.2
-
ethyl 3-hydroxybenzoate pH 7.0, 37°C Aspergillus nomiae
1.3
-
4-nitrophenyl acetate pH 7.0, 37°C Aspergillus nomiae
2.3
-
ethyl 3-methoxybenzoate pH 7.0, 37°C Aspergillus nomiae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion to the medium Aspergillus nomiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by EDTA, Ca2+, Mg2+, Co2+, Fe3+, Zn2+ Aspergillus nomiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Aspergillus nomiae

Organism

Organism UniProt Comment Textmining
Aspergillus nomiae
-
-
-
Aspergillus nomiae HS-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from culture medium to homogeneity, 28fold Aspergillus nomiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
135
-
purified enzyme, substrate ethyl benzoate Aspergillus nomiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Aspergillus nomiae 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Aspergillus nomiae HS-1 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Aspergillus nomiae 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Aspergillus nomiae HS-1 4-nitrophenol + butyrate
-
?
4-nitrophenyl caproate + H2O
-
Aspergillus nomiae 4-nitrophenol + caproate
-
?
4-nitrophenyl propionate + H2O
-
Aspergillus nomiae 4-nitrophenol + propionate
-
?
4-nitrophenyl propionate + H2O
-
Aspergillus nomiae HS-1 4-nitrophenol + propionate
-
?
4-nitrophenyl valerate + H2O
-
Aspergillus nomiae 4-nitrophenol + valerate
-
?
ethyl 3-hydroxybenzoate + H2O
-
Aspergillus nomiae ethanol + 3-hydroxybenzoate
-
?
ethyl 3-methoxybenzoate + H2O
-
Aspergillus nomiae ethanol + 3-methoxybenzoate
-
?
ethyl 4-hydroxybenzoate + H2O
-
Aspergillus nomiae ethanol + 4-hydroxybenzoate
-
?
ethyl 4-methoxybenzoate + H2O
-
Aspergillus nomiae ethanol + 4-methoxybenzoate
-
?
ethyl benzoate + H2O
-
Aspergillus nomiae ethanol + benzoate
-
?
ethylene glycol dibenzoate + H2O
-
Aspergillus nomiae ?
-
?
ethylene glycol dibenzoate + H2O
-
Aspergillus nomiae HS-1 ?
-
?
additional information dramatic decline in activity with substrates of chain length above C6, no activity with ethyl esters of 2-hydroxy- and 2-methoxybenzoates, phenyl acetate, ferulate, and diethyl esters of terephthalate and phthalate Aspergillus nomiae ?
-
?
additional information dramatic decline in activity with substrates of chain length above C6, no activity with ethyl esters of 2-hydroxy- and 2-methoxybenzoates, phenyl acetate, ferulate, and diethyl esters of terephthalate and phthalate Aspergillus nomiae HS-1 ?
-
?
n-butyl benzoate + H2O
-
Aspergillus nomiae n-butanol + benzoate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Aspergillus nomiae

Synonyms

Synonyms Comment Organism
carboxyl esterase
-
Aspergillus nomiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus nomiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
15 min, pH 7.0, loss of 5% activity Aspergillus nomiae
50
-
15 min, pH 7.0, loss of 55% activity Aspergillus nomiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Aspergillus nomiae

pH Range

pH Minimum pH Maximum Comment Organism
4 9 80% activity at pH 4.0 and pH 9.0 Aspergillus nomiae

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus nomiae isoelectric focusing
-
4.8