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Literature summary for 3.1.1.1 extracted from

  • Honda, K.; Kataoka, M.; Ono, H.; Sakamoto, K.; Kita, S.; Shimizu, S.
    Purification and characterization of a novel esterase promising for the production of useful compounds from Microbacterium sp. 7-1W (2002), FEMS Microbiol. Lett., 206, 221-227.
    View publication on PubMed
Search for more literature for 3.1.1.1

Application

Application Comment Organism
synthesis enzyme is useful for deprotection of the compounds in the production of cetraxate and D-panothenate Microbacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline
-
 Microbacterium sp.
diisopropyl phosphofluoridate
-
 Microbacterium sp.
HgCl2 slight inhibition Microbacterium sp.
additional information no inhibition by NaCl, KCl, MgCl2, MnCl2, CoCl2, NiCl2 Microbacterium sp.
N-ethylmaleimide slight inhibition Microbacterium sp.
PMSF
-
 Microbacterium sp.
Sodium cyanide poor inhibition Microbacterium sp.
ZnCl2 slight inhibition Microbacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 ethyl cetraxate pH 7.4, 30°C Microbacterium sp.
0.17
-
 isopropyl cetraxate pH 7.4, 30°C Microbacterium sp.
0.38
-
 methyl cetraxate pH 7.4, 30°C Microbacterium sp.
2.49
-
 methyl 3-(4-hydroxyphenyl)propionate pH 7.4, 30°C Microbacterium sp.
10
-
 n-propyl D-pantothenate pH 7.4, 30°C Microbacterium sp.
12.1
-
 n-propyl L-pantothenate pH 7.4, 30°C Microbacterium sp.
16.7
-
 ethyl D-pantothenate pH 7.4, 30°C Microbacterium sp.
18.3
-
 ethyl L-pantothenate pH 7.4, 30°C Microbacterium sp.
29.4
-
 methyl D-pantothenate pH 7.4, 30°C Microbacterium sp.
105
-
 methyl L-pantothenate pH 7.4, 30°C Microbacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Microbacterium sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 n * 62000, SDS-PAGE Microbacterium sp.
600000
-
 gel filtration Microbacterium sp.

Organism

Organism UniProt Comment Textmining
Microbacterium sp.
-
 AKU 163
-
Microbacterium sp. 7-1W
-
 AKU 163
-

Purification (Commentary)

Purification (Comment) Organism
solubilization with Brij58, 13.8fold to apparent homogeneity Microbacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
 purified enzyme Microbacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. benzyl cetraxate Microbacterium sp. benzol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
 ?
ethyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. ethyl cetraxate Microbacterium sp. ethanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
 ?
ethyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. ethyl cetraxate Microbacterium sp. 7-1W ethanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
 ?
ethyl acetate + H2O
-
 Microbacterium sp. ethanol + acetate
-
 ?
ethyl acetate + H2O
-
 Microbacterium sp. 7-1W ethanol + acetate
-
 ?
ethyl butyrate + H2O
-
 Microbacterium sp. ethanol + butanoate
-
 ?
ethyl caproate + H2O
-
 Microbacterium sp. ethanol + caproate
-
 ?
ethyl cetraxate + H2O
-
 Microbacterium sp. ethanol + cetraxate
-
 ?
ethyl D-pantothenate + H2O
-
 Microbacterium sp. ethanol + D-pantothenate
-
 ?
ethyl L-pantothenate + H2O
-
 Microbacterium sp. ethanol + L-pantothenate
-
 ?
ethyl valerate + H2O
-
 Microbacterium sp. ethanol + valerate
-
 ?
isopropyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. propyl cetraxate Microbacterium sp. isopropanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
 ?
isopropyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. propyl cetraxate Microbacterium sp. 7-1W isopropanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
 ?
isopropyl cetraxate + H2O
-
 Microbacterium sp. isopropanol + cetraxate
-
 ?
methyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. methyl cetraxate Microbacterium sp. methanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
 ?
methyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. methyl cetraxate Microbacterium sp. 7-1W methanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
 ?
methyl 3-(4-hydroxyphenyl)propionate + H2O
-
 Microbacterium sp. methanol + 3-(4-hydroxyphenyl)propionate
-
 ?
methyl cetraxate + H2O
-
 Microbacterium sp. methanol + cetraxate
-
 ?
methyl D-pantothenate + H2O
-
 Microbacterium sp. methanol + D-pantothenate
-
 ?
methyl L-pantothenate + H2O
-
 Microbacterium sp. methanol + L-pantothenate
-
 ?
additional information very low activity with methyl or ethyl esters of amino acids Microbacterium sp. ?
-
 ?
additional information very low activity with methyl or ethyl esters of amino acids Microbacterium sp. 7-1W ?
-
 ?
n-propyl D-pantothenate + H2O
-
 Microbacterium sp. n-propanol + D-pantothenate
-
 ?
n-propyl L-pantothenate + H2O best substrate Microbacterium sp. n-propanol + L-pantothenate
-
 ?

Subunits

Subunits Comment Organism
oligomer n * 62000, SDS-PAGE Microbacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 with substrate methyl cetraxate Microbacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 below, 30 min stable, pH 7.0 Microbacterium sp.
40
-
 30 min, pH 7.0, 13% remaining activity Microbacterium sp.
45
-
 30 min, pH 7.0, 9% remaining activity Microbacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 about Microbacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Microbacterium sp. isoelectric focusing
-
 4