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Literature summary for 3.1.1.1 extracted from

  • Junge, W.; Heymann, E.
    Characterization of the isoenzymes of pig-liver esterase. 2. Kinetic studies (1979), Eur. J. Biochem., 95, 519-525.
    View publication on PubMed
Search for more literature for 3.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
acetonitrile activates isoenzyme I, no increase in activity with isoenzyme V Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
acetone above 0.5 M Sus scrofa
bis(4-nitrophenyl) phosphate 0.01 mM, 30 min, 89% loss of activity of isoenzyme V, 97% loss of activity of isoenzyme I Sus scrofa
NaF 1 mM, 30 min, 55% loss of activity of isoenzyme I, 2% loss of activity of isoenzyme V Sus scrofa
paraoxon 0.001 mM, 30 min, complete inhibition of isoenzyme V, 95% loss of activity of isoenzyme I Sus scrofa
physostigmine 0.01 mM, 3 min, no loss of activity of isoenzyme V, 95% loss of activity of isoenzyme I Sus scrofa
PMSF 0.01 mM, 30 min, 94% loss of activity of isoenzyme V, 27% loss of activity of isoenzyme I Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 methyl octanoate isoenzyme V Sus scrofa
0.043
-
 4-nitrophenyl acetate isoenzyme V Sus scrofa
0.05
-
 phenyl acetate isoenzyme V Sus scrofa
0.22
-
 4-nitrophenyl acetate isoenzyme I Sus scrofa
0.4
-
 methyl octanoate isoenzyme I Sus scrofa
0.43
-
 phenyl acetate isoenzyme I Sus scrofa
0.6
-
 butanilicain isoenzyme V Sus scrofa
1.6
-
 butanilicain isoenzyme I Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 isoenzyme I and V
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Sus scrofa 4-nitrophenol + acetate
-
 ?
acetanilide + H2O
-
 Sus scrofa acetate + aniline
-
 ?
butanilicain + H2O
-
 Sus scrofa ?
-
 ?
butanilicain + H2O i.e. N-butylglycyl-2-chloro-6-methylanilide Sus scrofa ?
-
 ?
butyrylthiocholine + H2O
-
 Sus scrofa butyric acid + thiocholine
-
 ?
estrone acetate + H2O
-
 Sus scrofa estrone + acetate
-
 ?
methyl butyrate + H2O
-
 Sus scrofa methanol + butyrate
-
 ?
methyl hexanoate + H2O
-
 Sus scrofa methanol + hexanoate
-
 ?
methyl octanoate + H2O
-
 Sus scrofa methanol + octanoate
-
 ?
N-ethylglycine xylidide + H2O
-
 Sus scrofa ?
-
 ?
phenyl acetate + H2O
-
 Sus scrofa phenol + acetate
-
 ?
tributyrylglycerol + H2O
-
 Sus scrofa butyrate + glycerol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 hydrolysis of methyl hexanoate, isoenzyme V Sus scrofa
8
-
 hydrolysis of methyl hexanoate, isoenzyme I Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 about 60% of maximal activity at pH 5.5 and at pH 9.0, hydrolysis of methyl hexanoate, isoenzyme V Sus scrofa
5.5 9.5 pH 5.5: about 45% of maximal activity, pH 9.5: about 80% of maximal activity, hydrolysis of methyl hexanoate, isoenzyme I Sus scrofa