Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.9.1.1 extracted from

  • Manzine, L.; Cassago, A.; Da Silva, M.; Thiemann, O.
    An efficient protocol for the production of tRNA-free recombinant selenocysteine synthase (SELA) from Escherichia coli and its biophysical characterization (2013), Protein Expr. Purif., 88, 80-84.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain WL81460 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
-
Escherichia coli
600000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-seryl-tRNASec + selenophosphate Escherichia coli
-
L-selenocysteinyl-tRNASec + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydroxyapatite column chromatography, Hi Trap Q column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-seryl-tRNASec + selenophosphate
-
Escherichia coli L-selenocysteinyl-tRNASec + phosphate
-
?

Subunits

Subunits Comment Organism
homodecamer 10 * 50000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
SelA
-
Escherichia coli
Selenocysteine synthase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
the protein stability is maintained up to 45°C. The enzyme has a thermal transition from 60 to 70°C with a melting temperature of 66°C and an evident plateau above 70°C, in which all secondary structures are lost and the protein is completely denatured Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
6