Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain WL81460 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
- |
Escherichia coli |
600000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-seryl-tRNASec + selenophosphate | Escherichia coli | - |
L-selenocysteinyl-tRNASec + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, hydroxyapatite column chromatography, Hi Trap Q column chromatography, and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-seryl-tRNASec + selenophosphate | - |
Escherichia coli | L-selenocysteinyl-tRNASec + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodecamer | 10 * 50000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
SelA | - |
Escherichia coli |
Selenocysteine synthase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the protein stability is maintained up to 45°C. The enzyme has a thermal transition from 60 to 70°C with a melting temperature of 66°C and an evident plateau above 70°C, in which all secondary structures are lost and the protein is completely denatured | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
6 |