BRENDA - Enzyme Database
show all sequences of 2.9.1.1

Structure of selenocysteine synthase from Escherichia coli and location of tRNA in the seryl-tRNASec-enzyme complex

Engelhardt, H.; Forchhammer, K.; Muller, S.; Goldie, K.N.; Böck, A.; Mol. Microbiol. 6, 3461-3467 (1992)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
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Reaction
Reaction
Commentary
Organism
L-seryl-tRNASec + selenophosphate = L-selenocysteinyl-tRNASec + phosphate
tRNA is bound near the margin of the dimeric subunits, appears to bind only one seryl-tRNA Sec per dimer
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Seryl-tRNASec + selenophosphate
-
22915
Escherichia coli
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
22915
Escherichia coli
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Seryl-tRNASec + selenophosphate
-
22915
Escherichia coli
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
22915
Escherichia coli
-
Other publictions for EC 2.9.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738683
Manhas
Leishmania donovani encodes a ...
Leishmania donovani
J. Biol. Chem.
291
1203-1220
2016
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725024
Manzine
Assembly stoichiometry of bact ...
Escherichia coli
FEBS Lett.
587
906-911
2013
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739586
Manzine
An efficient protocol for the ...
Escherichia coli
Protein Expr. Purif.
88
80-84
2013
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739706
Itoh
Decameric SelA-tRNA(Sec) ring ...
Aquifex aeolicus
Science
340
75-78
2013
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693212
Ganichkin
Structure and catalytic mechan ...
Mus musculus
J. Biol. Chem.
283
5849-5865
2008
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691146
Fischer
Towards understanding selenocy ...
Moorella thermoacetica
Biol. Chem.
388
1061-1067
2007
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691179
Xu
New developments in selenium b ...
Homo sapiens, Mus musculus
Biol. Trace Elem. Res.
119
234-241
2007
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660828
Gursinsky
A selDABC cluster for selenocy ...
Peptoclostridium acidaminophilum
Arch. Microbiol.
174
200-212
2000
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661290
Mizutani
Selenophosphate as a substrate ...
Bos taurus
Biofactors
9
27-36
1999
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22917
Tormay
Bacterial selenocysteine synth ...
Desulfomicrobium baculatum, Escherichia coli, Haemophilus influenzae, Moorella thermoacetica
Eur. J. Biochem.
254
655-661
1998
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22916
Tanabe
-
Selenophosphate as a substrate ...
Bos taurus
Nucleic acids Symp. Ser.
35
287-288
1996
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22915
Engelhardt
Structure of selenocysteine sy ...
Escherichia coli
Mol. Microbiol.
6
3461-3467
1992
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22912
Forchhammer
Selenocysteine synthase from E ...
Escherichia coli
J. Biol. Chem.
266
6324-6328
1991
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22913
Forchhammer
Selenocysteine synthase from E ...
Escherichia coli
J. Biol. Chem.
266
6318-6323
1991
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22914
Forchhammer
The function of selenocysteine ...
Escherichia coli
Biochimie
73
1481-1486
1991
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