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Literature summary for 2.8.4.6 extracted from
- 1-methylthio-d-xylulose 5-phosphate methylsulfurylase A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum (2012), Biochemistry, 51, 8324-8326 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C121A | inactive | Rhodospirillum rubrum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.039 | - |
L-dithiothreitol | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum | |
| 0.042 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum |  |
| 0.46 | - |
dihydrolipoate | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-methyl-1-thio-D-xylulose 5-phosphate + glutathione | Rhodospirillum rubrum | - |
1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione | - |
? | |
| S-methyl-1-thio-D-xylulose 5-phosphate + glutathione | Rhodospirillum rubrum DSM 467 | - |
1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | Q2RSU5 | - |
- |
| Rhodospirillum rubrum DSM 467 | Q2RSU5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | in vivo, acceptor is glutathione, in vitro, glutathione can be substituted by dithiothreitol or dihydrolipoate | Rhodospirillum rubrum | ? | - |
- |
|
| additional information | in vivo, acceptor is glutathione, in vitro, glutathione can be substituted by dithiothreitol or dihydrolipoate | Rhodospirillum rubrum DSM 467 | ? | - |
- |
|
| S-methyl-1-thio-D-xylulose 5-phosphate + dihydrolipoate | - |
Rhodospirillum rubrum | 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dihydrolipoate | - |
? | |
| S-methyl-1-thio-D-xylulose 5-phosphate + dihydrolipoate | - |
Rhodospirillum rubrum DSM 467 | 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dihydrolipoate | - |
? | |
| S-methyl-1-thio-D-xylulose 5-phosphate + glutathione | - |
Rhodospirillum rubrum | 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione | - |
? | |
| S-methyl-1-thio-D-xylulose 5-phosphate + glutathione | - |
Rhodospirillum rubrum DSM 467 | 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione | - |
? | |
| S-methyl-1-thio-D-xylulose 5-phosphate + L-dithiothreitol | - |
Rhodospirillum rubrum | 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dithiothreitol | - |
? | |
| S-methyl-1-thio-D-xylulose 5-phosphate + L-dithiothreitol | - |
Rhodospirillum rubrum DSM 467 | 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dithiothreitol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MTXu 5-P methylsulfurylase | - |
Rhodospirillum rubrum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum | |
| 4.3 | - |
dihydrolipoate | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum | |
| 7.8 | - |
L-dithiothreitol | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | an active site Cys residue displaces the enolate of 1-deoxy-D-xylulose 5-phosphate from 1-methylthio-D-xylulose 5-phosphate, generating a methyl disulfide intermediate | Rhodospirillum rubrum |
| physiological function | enzyme belongs to the cupin superfamily and participates in an anaerobic salvage pathway that restores methionine from S-methyl-5'-thioadenosine | Rhodospirillum rubrum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum | |
| 93 | - |
dihydrolipoate | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum | |
| 200 | - |
L-dithiothreitol | pH not specified in the publication, temperature not specified in the publication | Rhodospirillum rubrum |
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