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Literature summary for 2.8.4.3 extracted from

  • Landgraf, B.J.; Arcinas, A.J.; Lee, K.H.; Booker, S.J.
    Identification of an intermediate methyl carrier in the radical S-adenosylmethionine methylthiotransferases RimO and MiaB (2013), J. Am. Chem. Soc., 135, 15404-15416.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.4.3

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WZC1
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Thermotoga maritima DSM 3109 Q9WZC1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes methyl transfer from S-adenosyl-L-methionine to an acid/base labile acceptor on the protein in the absence of their respective macromolecular substrates. Denaturation of the S-adenosyl-L-methionine-treated protein with acid results in production of ethanethiol. When the enzyme is first incubated with S-adenosyl-L-methionine in the absence of substrate and reductant and then incubated with excess S-adenosyl-L-[methyl-D3]methionine in the presence of substrate and reductant, production of the unlabeled product precedes production of the deuterated product, showing that the methylated species is chemically and kinetically competent to be an intermediate Thermotoga maritima ?
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additional information enzyme catalyzes methyl transfer from S-adenosyl-L-methionine to an acid/base labile acceptor on the protein in the absence of their respective macromolecular substrates. Denaturation of the S-adenosyl-L-methionine-treated protein with acid results in production of ethanethiol. When the enzyme is first incubated with S-adenosyl-L-methionine in the absence of substrate and reductant and then incubated with excess S-adenosyl-L-[methyl-D3]methionine in the presence of substrate and reductant, production of the unlabeled product precedes production of the deuterated product, showing that the methylated species is chemically and kinetically competent to be an intermediate Thermotoga maritima DSM 3109 ?
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N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine
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 Thermotoga maritima 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?
N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine
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 Thermotoga maritima DSM 3109 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?