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Literature summary for 2.8.4.2 extracted from

  • Ordonez, E.; Van Belle, K.; Roos, G.; De Galan, S.; Letek, M.; Gil, J.A.; Wyns, L.; Mateos, L.M.; Messens, J.
    Arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange (2009), J. Biol. Chem., 284, 15107-15116.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.4.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information gene deletion mutants of isoforms ArsC1 and ArsC2 show similar resistance levels to As(V) as wild-type. the ArsC1/ArsC2 double mutant strain is very sensitive to arsenate and shows resistance levels, which are 20–30 times lower. Only plasmids containing either the arsC1 or the arsC2 gene are able to complement the arsenate reductase activity. When the single cysteine in the arsC1 and the arsC2 genes is mutated to a serine, the complementation is lost. Removing arsC1, arsC2, or both genes had no effect on the resistance against As(III), and resistance levels equivalent to those for wild type are obtained Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 arsenate isoform ArsC2, pH 8.0, presence of phosphate Corynebacterium glutamicum
0.038
-
 arsenate isoform ArsC2, pH 8.0, presence of sulfate Corynebacterium glutamicum
0.046
-
 arsenate isoform ArsC1, pH 8.0, presence of sulfate Corynebacterium glutamicum
0.082
-
 arsenate isoform ArsC2, pH 8.0 Corynebacterium glutamicum
0.13
-
 arsenate isoform ArsC1, pH 8.0, presence of phosphate Corynebacterium glutamicum
0.142
-
 arsenate isoform ArsC1, pH 8.0 Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arsenate + mycothiol Corynebacterium glutamicum arsenate reductases (Cg_ArsC1 and Cg_ArsC2) are part of a defense mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by mycoredoxin (Mrx1) to arsenite and mycothiol-mycoredoxin disulfide. Finally a second mycothiol recycles mycoredoxin and forms mycothione arseno-mycothiol
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ArsC1 and ArsC2 Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenate + mycothiol arsenate reductases (Cg_ArsC1 and Cg_ArsC2) are part of a defense mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by mycoredoxin (Mrx1) to arsenite and mycothiol-mycoredoxin disulfide. Finally a second mycothiol recycles mycoredoxin and forms mycothione Corynebacterium glutamicum arseno-mycothiol
-
 ?
arsenate + mycothiol reduction of arsenate is part of a defense mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by mycoredoxin (Mrx1) to arsenite and mycothiol-mycoredoxin disulfide. Finally a second mycothiol recycles mycoredoxin and forms mycothione Corynebacterium glutamicum arseno-mycothiol
-
 ?

Synonyms

Synonyms Comment Organism
Cg_ArsC1
-
 Corynebacterium glutamicum
Cg_ArsC2
-
 Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 arsenate isoform ArsC2, pH 8.0, presence of sulfate Corynebacterium glutamicum
0.17
-
 arsenate isoform ArsC2, pH 8.0, presence of phosphate Corynebacterium glutamicum
0.23
-
 arsenate isoform ArsC1, pH 8.0, presence of sulfate Corynebacterium glutamicum
0.28
-
 arsenate isoform ArsC2, pH 8.0 Corynebacterium glutamicum
0.53
-
 arsenate isoform ArsC1, pH 8.0 Corynebacterium glutamicum
0.6
-
 arsenate isoform ArsC1, pH 8.0, presence of phosphate Corynebacterium glutamicum