Cloned (Comment) | Organism |
---|---|
genetic organization of the mcr operon, overview | Methanosarcina acetivorans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | methylation mutant Mko4551 shows impaired growth under stress conditions | Methanosarcina acetivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | contained in the coenzyme F430 | Methanosarcina acetivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl-CoM + CoB | Methanosarcina acetivorans | - |
CoM-S-S-CoB + methane | - |
? | |
methyl-CoM + CoB | Methanosarcina acetivorans ATCC 35395 | - |
CoM-S-S-CoB + methane | - |
? | |
methyl-CoM + CoB | Methanosarcina acetivorans DSM 2834 | - |
CoM-S-S-CoB + methane | - |
? | |
methyl-CoM + CoB | Methanosarcina acetivorans JCM 12185 | - |
CoM-S-S-CoB + methane | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | Q8THH1 AND Q8THG7 AND Q8THH0 | subunits A, B, and G; WWM1 and Mko4551 genotypes | - |
Methanosarcina acetivorans ATCC 35395 | Q8THH1 AND Q8THG7 AND Q8THH0 | subunits A, B, and G; WWM1 and Mko4551 genotypes | - |
Methanosarcina acetivorans DSM 2834 | Q8THH1 AND Q8THG7 AND Q8THH0 | subunits A, B, and G; WWM1 and Mko4551 genotypes | - |
Methanosarcina acetivorans JCM 12185 | Q8THH1 AND Q8THG7 AND Q8THH0 | subunits A, B, and G; WWM1 and Mko4551 genotypes | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
alkylation | enzyme MCR contains methylhistidine (His271) and methylcysteine (Cys472), as well as 5-C-(S)-methylarginine. A unique radical SAM methyltransferase is required for the sp3-C-methylation of the arginine residue of methyl-coenzyme M reductase, the a 5-C-(S)-methylarginine is located close to the active site of the enzyme. Deletion of the methyltransferase gene ma4551 in Methanosarcina acetivorans strain WWM1 leads to the production of an active MCR lacking the C-5-methylation of the respective arginine residue. The methylated arginine is important for MCR stability under stress conditions Identification of potential radical SAM methyltransferase genes in the genomes of methanogens, overview | Methanosarcina acetivorans |
additional information | four amino acid modifications are reported to be present in McrA, namely methylhistidine (His271), methylcysteine (Cys472), thioglycine (Gly465) and didehydroaspartate (Asp470). Mass spectrometric analysis | Methanosarcina acetivorans |
side-chain modification | the enzyme contains thioglycine (Gly465) and didehydroaspartate (Asp470) | Methanosarcina acetivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl-CoM + CoB | - |
Methanosarcina acetivorans | CoM-S-S-CoB + methane | - |
? | |
methyl-CoM + CoB | - |
Methanosarcina acetivorans ATCC 35395 | CoM-S-S-CoB + methane | - |
? | |
methyl-CoM + CoB | - |
Methanosarcina acetivorans DSM 2834 | CoM-S-S-CoB + methane | - |
? | |
methyl-CoM + CoB | - |
Methanosarcina acetivorans JCM 12185 | CoM-S-S-CoB + methane | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MCR | - |
Methanosarcina acetivorans |
mcrA | - |
Methanosarcina acetivorans |
mcrA | subunit A | Methanosarcina acetivorans |
mcrB | - |
Methanosarcina acetivorans |
mcrB | subunit B | Methanosarcina acetivorans |
mcrG | - |
Methanosarcina acetivorans |
mcrG | subunit G | Methanosarcina acetivorans |
methyl-coenzyme M reductase | - |
Methanosarcina acetivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
F-430 | coenzyme F430 | Methanosarcina acetivorans |
General Information | Comment | Organism |
---|---|---|
malfunction | methylation mutant Mko4551 shows impaired growth under stress conditions | Methanosarcina acetivorans |
metabolism | the last and methane-releasing step of methanogenesis is catalysed by the enzyme methyl-coenzyme M reductase (MCR) | Methanosarcina acetivorans |