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Literature summary for 2.8.4.1 extracted from

  • Deobald, D.; Adrian, L.; Sch๖ne, C.; Rother, M.; Layer, G.
    Identification of a unique Radical SAM methyltransferase required for the sp3-C-methylation of an arginine residue of methyl-coenzyme M reductase (2018), Sci. Rep., 8, 7404 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genetic organization of the mcr operon, overview Methanosarcina acetivorans

Protein Variants

Protein Variants Comment Organism
additional information methylation mutant Mko4551 shows impaired growth under stress conditions Methanosarcina acetivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ contained in the coenzyme F430 Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl-CoM + CoB Methanosarcina acetivorans
-
CoM-S-S-CoB + methane
-
?
methyl-CoM + CoB Methanosarcina acetivorans ATCC 35395
-
CoM-S-S-CoB + methane
-
?
methyl-CoM + CoB Methanosarcina acetivorans DSM 2834
-
CoM-S-S-CoB + methane
-
?
methyl-CoM + CoB Methanosarcina acetivorans JCM 12185
-
CoM-S-S-CoB + methane
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8THH1 AND Q8THG7 AND Q8THH0 subunits A, B, and G; WWM1 and Mko4551 genotypes
-
Methanosarcina acetivorans ATCC 35395 Q8THH1 AND Q8THG7 AND Q8THH0 subunits A, B, and G; WWM1 and Mko4551 genotypes
-
Methanosarcina acetivorans DSM 2834 Q8THH1 AND Q8THG7 AND Q8THH0 subunits A, B, and G; WWM1 and Mko4551 genotypes
-
Methanosarcina acetivorans JCM 12185 Q8THH1 AND Q8THG7 AND Q8THH0 subunits A, B, and G; WWM1 and Mko4551 genotypes
-

Posttranslational Modification

Posttranslational Modification Comment Organism
alkylation enzyme MCR contains methylhistidine (His271) and methylcysteine (Cys472), as well as 5-C-(S)-methylarginine. A unique radical SAM methyltransferase is required for the sp3-C-methylation of the arginine residue of methyl-coenzyme M reductase, the a 5-C-(S)-methylarginine is located close to the active site of the enzyme. Deletion of the methyltransferase gene ma4551 in Methanosarcina acetivorans strain WWM1 leads to the production of an active MCR lacking the C-5-methylation of the respective arginine residue. The methylated arginine is important for MCR stability under stress conditions Identification of potential radical SAM methyltransferase genes in the genomes of methanogens, overview Methanosarcina acetivorans
additional information four amino acid modifications are reported to be present in McrA, namely methylhistidine (His271), methylcysteine (Cys472), thioglycine (Gly465) and didehydroaspartate (Asp470). Mass spectrometric analysis Methanosarcina acetivorans
side-chain modification the enzyme contains thioglycine (Gly465) and didehydroaspartate (Asp470) Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl-CoM + CoB
-
Methanosarcina acetivorans CoM-S-S-CoB + methane
-
?
methyl-CoM + CoB
-
Methanosarcina acetivorans ATCC 35395 CoM-S-S-CoB + methane
-
?
methyl-CoM + CoB
-
Methanosarcina acetivorans DSM 2834 CoM-S-S-CoB + methane
-
?
methyl-CoM + CoB
-
Methanosarcina acetivorans JCM 12185 CoM-S-S-CoB + methane
-
?

Synonyms

Synonyms Comment Organism
MCR
-
Methanosarcina acetivorans
mcrA
-
Methanosarcina acetivorans
mcrA subunit A Methanosarcina acetivorans
mcrB
-
Methanosarcina acetivorans
mcrB subunit B Methanosarcina acetivorans
mcrG
-
Methanosarcina acetivorans
mcrG subunit G Methanosarcina acetivorans
methyl-coenzyme M reductase
-
Methanosarcina acetivorans

Cofactor

Cofactor Comment Organism Structure
F-430 coenzyme F430 Methanosarcina acetivorans

General Information

General Information Comment Organism
malfunction methylation mutant Mko4551 shows impaired growth under stress conditions Methanosarcina acetivorans
metabolism the last and methane-releasing step of methanogenesis is catalysed by the enzyme methyl-coenzyme M reductase (MCR) Methanosarcina acetivorans