Literature summary for 2.8.4.1 extracted from

Nayak, D.D.; Liu, A.; Agrawal, N.; Rodriguez-Carerro, R.; Dong, S.H.; Mitchell, D.A.; Nair, S.K.; Metcalf, W.W.
Functional interactions between posttranslationally modified amino acids of methyl-coenzyme M reductase in Methanosarcina acetivorans (2020), PLoS Biol., 18, e3000507 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	generation of side-chain mutants to gain molecular insights into the nature of the S-methylcysteine, thioglycine, and 5-(S)-methylarginine interactions and their influence on the activity and stability of MCR, structure-function analysis. The local structure near Gly465 (modified to ThioGly), Cys472 (modified to MeCys), and Arg285 (modified to MeArg) in wild type are compared to the local structure near the unmodified Gly465 residue, unmodified Cys472 residue, and unmodified Arg285 residue in MCR derived from the DELTAycaO-tfuA, DELTAmcmA, and DELTAmamA single mutants, respectively	Methanosarcina acetivorans

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina acetivorans	Q8THH1	-	-
Methanosarcina acetivorans ATCC 35395	Q8THH1	-	-
Methanosarcina acetivorans DSM 2834	Q8THH1	-	-
Methanosarcina acetivorans JCM 12185	Q8THH1	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
alkylation	S-methylation of Cys472 in McrA by a SAM-dependent methyltransferase MA4545 or McmA belonging to the InterPro superfamily (UniProt ID Q8THH2). The DELTAmcmA mutant is viable on all growth substrates tested, relative to wild-type, the mutant grows 30% slower on DMS, and a 12% decrease in growth yield is observed on trimethylamine. Even though the S-methylation of Cys472 in McrA is dispensable in Methanosarcina acetivorans, it is clearly important for methanogenic growth on certain substrates. Conversion of Arg285 in McrA to 5-(S)-methylarginine depends on enzyme MamA. The L461-R491 peptide contains the Gly465 and Cys472 that are modified to thioglycine and S-methylcysteine by ycaO-tfuA and mcmA, respectively, as well as the didehydroaspartate at Asp470	Methanosarcina acetivorans
additional information	functional interactions between posttranslationally modified amino acids of methyl-coenzyme M reductase, overview. Modified McrA residues are independently installed	Methanosarcina acetivorans
side-chain modification	the L461-R491 peptide contains the Gly465 and Cys472 that are modified to thioglycine and S-methylcysteine by ycaO-tfuA (MA0165/MA0164) and mcmA, respectively, as well as the didehydroaspartate at Asp470	Methanosarcina acetivorans

Subunits

Subunits	Comment	Organism
heterotrimer	-	Methanosarcina acetivorans

Synonyms

Synonyms	Comment	Organism
MaMCR	-	Methanosarcina acetivorans
mcrA	subunit A	Methanosarcina acetivorans

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Release 2023.1 (January 2023)