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Literature summary for 2.8.4.1 extracted from

  • Nayak, D.; Mahanta, N.; Mitchell, D.; Metcalf, W.
    Post-translational thioamidation of methyl-coenzyme M reductase, a key enzyme in methanogenic and methanotrophic Archaea (2017), eLife, 6, e29218 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.4.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of Strep-tagged enzyme Methanosarcina barkeri
recombinant expression of Strep-tagged enzyme Methanosarcina acetivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ contained in the coenzyme F430 Methanosarcina barkeri
Ni2+ contained in the coenzyme F430 Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl-CoM + CoB Methanosarcina barkeri
-
 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB Methanosarcina acetivorans
-
 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB Methanosarcina acetivorans ATCC 35395
-
 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB Methanosarcina barkeri Fusaro
-
 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB Methanosarcina acetivorans DSM 2834
-
 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB Methanosarcina acetivorans JCM 12185
-
 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB Methanosarcina barkeri DSM 804
-
 CoM-S-S-CoB + methane
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8THH1 AND Q8THG7 AND Q8THH0 subunits alpha, beta, and gamma
-
Methanosarcina acetivorans ATCC 35395 Q8THH1 AND Q8THG7 AND Q8THH0 subunits alpha, beta, and gamma
-
Methanosarcina acetivorans DSM 2834 Q8THH1 AND Q8THG7 AND Q8THH0 subunits alpha, beta, and gamma
-
Methanosarcina acetivorans JCM 12185 Q8THH1 AND Q8THG7 AND Q8THH0 subunits alpha, beta, and gamma
-
Methanosarcina barkeri P07962 and P07955 and P07964 subunits alpha, beta, and gamma
-
Methanosarcina barkeri DSM 804 P07962 and P07955 and P07964 subunits alpha, beta, and gamma
-
Methanosarcina barkeri Fusaro P07962 and P07955 and P07964 subunits alpha, beta, and gamma
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information hylogenetic analyses of methyltransferases TfuA and YcaO in methanogenic andmethanotrophic archaea Methanosarcina acetivorans
side-chain modification formation of thioglycine (Gly465), growth defects are associated with loss of the McrA thioglycine modification, mutations in ycaO and/or tfuA are responsible for loss of the thioglycine modification. Thioglycine does not influence the global stability of MCR Methanosarcina acetivorans

Purification (Commentary)

Purification (Comment) Organism
recombinant Strep-tagged enzyme by affinity chromatography Methanosarcina barkeri
recombinant Strep-tagged enzyme by affinity chromatography Methanosarcina acetivorans

Reaction

Reaction Comment Organism Reaction ID
methyl-CoM + CoB = CoM-S-S-CoB + methane the reaction proceeds via an unprecedented methyl radical intermediate Methanosarcina barkeri
a
methyl-CoM + CoB = CoM-S-S-CoB + methane the reaction proceeds via an unprecedented methyl radical intermediate Methanosarcina acetivorans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl-CoM + CoB
-
 Methanosarcina barkeri CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB
-
 Methanosarcina acetivorans CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB
-
 Methanosarcina acetivorans ATCC 35395 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB
-
 Methanosarcina barkeri Fusaro CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB
-
 Methanosarcina acetivorans DSM 2834 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB
-
 Methanosarcina acetivorans JCM 12185 CoM-S-S-CoB + methane
-
 ?
methyl-CoM + CoB
-
 Methanosarcina barkeri DSM 804 CoM-S-S-CoB + methane
-
 ?

Synonyms

Synonyms Comment Organism
MCR
-
 Methanosarcina barkeri
MCR
-
 Methanosarcina acetivorans
methyl-coenzyme M reductase
-
 Methanosarcina barkeri
methyl-coenzyme M reductase
-
 Methanosarcina acetivorans

Cofactor

Cofactor Comment Organism Structure
F-430 coenzyme F430 Methanosarcina barkeri
F-430 coenzyme F430 Methanosarcina acetivorans

General Information

General Information Comment Organism
malfunction growth defects associated with loss of the McrA thioglycine modification. The DELTAycaO-tfuA mutant lacks the McrA Gly465 thioamide Methanosarcina acetivorans
physiological function methyl-coenzyme M reductase is a key enzyme in methanogenic and methanotrophic Archaea. Methyl-coenzyme M reductase (MCR) is a unique enzyme found exclusively in anaerobic archaea, where it catalyzes the reversible conversion of methyl-coenzyme M (CoM, 2-methylmercaptoethane-sulfonate) and coenzyme B (CoB, 7-thioheptanoylthreoninephosphate) to methane and a CoB-CoM heterodisulfide. The activity plays a critical role in the global carbon cycle Methanosarcina barkeri
physiological function methyl-coenzyme M reductase is a key enzyme in methanogenic and methanotrophic Archaea. Methyl-coenzyme M reductase (MCR) is a unique enzyme found exclusively in anaerobic archaea, where it catalyzes the reversible conversion of methyl-coenzyme M (CoM, 2-methylmercaptoethane-sulfonate) and coenzyme B (CoB, 7-thioheptanoylthreoninephosphate) to methane and a CoB-CoM heterodisulfide. The activity plays a critical role in the global carbon cycle Methanosarcina acetivorans