Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butanesulfonate | - |
Methanothermobacter marburgensis | |
1-propanesulfonate | - |
Methanothermobacter marburgensis | |
2-bromoethanesulfonate | - |
Methanothermobacter marburgensis | |
3-bromopropanesulfonate | irreversible, strong inhibitor and competitive substrate | Methanothermobacter marburgensis | |
3-Bromopropionate | - |
Methanothermobacter marburgensis | |
3-chloropropanesulfonyl chloride | - |
Methanothermobacter marburgensis | |
3-mercapto-1-propanesulfonate | - |
Methanothermobacter marburgensis | |
4-bromobutanesulfonate | - |
Methanothermobacter marburgensis | |
4-bromobutyrate | - |
Methanothermobacter marburgensis | |
4-Chlorobutyrate | - |
Methanothermobacter marburgensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state and rapid kinetics, MCRred1-catalyzed cleavage of the C-S bond of methyl-SCoM requires the other substrate, HSCoB, even under single turnover conditions | Methanothermobacter marburgensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl-coenzyme M + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) | Methanothermobacter marburgensis | i.e. methyl-SCoM | methane + CoB-S-S-CoM | a the mixed disulfide | ? | |
additional information | Methanothermobacter marburgensis | MCR also appears to initiate anaerobic methane oxidation, the reverse methanogenesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter marburgensis | - |
i.e. Methanothermobacter thermoautotrophicum strain Marburg | - |
Purification (Comment) | Organism |
---|---|
purification of native MCRox1, and of native MCRred1, the latter by ammonium sulfate fractionation and ion exchange chromatography | Methanothermobacter marburgensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methyl-CoM + CoB = CoM-S-S-CoB + methane | two possible catalytic mechanisms, overview | Methanothermobacter marburgensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl-coenzyme M + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) | i.e. methyl-SCoM | Methanothermobacter marburgensis | methane + CoB-S-S-CoM | a the mixed disulfide | ? | |
additional information | MCR also appears to initiate anaerobic methane oxidation, the reverse methanogenesis | Methanothermobacter marburgensis | ? | - |
? | |
additional information | conversion of MCRox1 toMCRred1 by Ti(III)citrate, bromopropanesulfonate is an alternative substrate of MCR in an ionic reaction that is coenzyme B-independent and leads to debromination of bromopropanesulfonate and formation of a distinct state with an EPR signal that is assigned to a Ni(III)-propylsulfonate species, propanesulfonate formation also occurs in steady-state reactions in the presence of Ti(III) citrate | Methanothermobacter marburgensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methyl-coenzyme M reductase | - |
Methanothermobacter marburgensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 25 | assay at | Methanothermobacter marburgensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 65 | - |
Methanothermobacter marburgensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH 10.0 is optimal for activation of MCRox1 to MCRred1 with Ti(III) citrate | Methanothermobacter marburgensis |
7 | 7.6 | assay at | Methanothermobacter marburgensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F430 | the nickel-containing tetrapyrrole is essential for the reaction, and is bound to the active site | Methanothermobacter marburgensis |