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Literature summary for 2.8.4.1 extracted from
- Reductive activation of the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum.DELTA.H (1988), J. Bacteriol., 170, 3946-3952.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | absolute requirement, activation of the methylreductase system and not at the catalytic level | Methanothermobacter thermautotrophicus |  |
| CN-B12 | - |
Methanothermobacter thermautotrophicus | |
| coenzyme F-420 | enzyme system is stimulated | Methanothermobacter thermautotrophicus | |
| dithiothreitol | - |
Methanothermobacter thermautotrophicus | |
| H2 | suggested to be involved in the activation of the methylreductase, preincubation | Methanothermobacter thermautotrophicus | |
| Ti(III) citrate | artificial electron donor, if used as electron source component A1 is no longer required | Methanothermobacter thermautotrophicus | |
| vitamin B12 | enzyme system is stimulated | Methanothermobacter thermautotrophicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
coenzyme B | - |
Methanothermobacter thermautotrophicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni(2+) | not detected | Methanothermobacter thermautotrophicus | |
| Ni2+ | - |
Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus | reductive methylation | methane + CoM-S-S-CoB | - |
? | |
| methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus DELTAH | reductive methylation | methane + CoM-S-S-CoB | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
deltaH | - |
| Methanothermobacter thermautotrophicus DELTAH | - |
deltaH | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anaerobic, different components purified seperately, component C ion-exchange chromatography, hydrophobic interaction chromatography, component A2 affinity chromatography, ion-exchange chromatography, component A1 and A3 are obtained by gel filtration, hydrophobic interaction chromatography | Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.9 | - |
for component C | Methanothermobacter thermautotrophicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, component C, 96% homogeneous, under N2 atmosphere, several months without any loss of activity in multicomponent assay | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl coenzyme M + coenzyme B | reductive methylation | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
| methyl coenzyme M + coenzyme B | reductive methylation | Methanothermobacter thermautotrophicus DELTAH | methane + CoM-S-S-CoB | - |
? | |
| methyl-coenzyme M + coenzyme B | methane formation only under H2 not under N2 atmosphere. ATP and FAD also required. Ti(III) citrate can be used as electron source under N2 atmosphere. Dithiothreitol and cyanocobalamin under H2 can also be used for methanogenesis | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
| methyl-coenzyme M + coenzyme B | methane formation only under H2 not under N2 atmosphere. ATP and FAD also required. Ti(III) citrate can be used as electron source under N2 atmosphere. Dithiothreitol and cyanocobalamin under H2 can also be used for methanogenesis | Methanothermobacter thermautotrophicus DELTAH | methane + CoM-S-S-CoB | - |
? | |
| additional information | dual requirement for electron donors | Methanothermobacter thermautotrophicus | ? | - |
? | |
| additional information | dual requirement for electron donors | Methanothermobacter thermautotrophicus DELTAH | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | four protein components: A1 (coenzyme F-420-reducing hydrogenase), A2, A3 (methylviologen-reducing hydrogenase) and C as well as catalytic amounts of ATP, vitamin B12 and the disulfide of 7-mercaptoheptanoylthreonine in addition to Ti(III) citrate, component A1 is no longer required, component A1 and A3 500000 Da from gel-filtration | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay | Methanothermobacter thermautotrophicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| F-430 | - |
Methanothermobacter thermautotrophicus | |
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