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Literature summary for 2.8.4.1 extracted from
- The final step in methane formation. Investigations with highly purified methyl-CoM reductase (component C) from Methanobacterium thermoautotrophicum (strain Marburg) (1988), Eur. J. Biochem., 172, 669-677.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cobalamin | aquocobalamin | Methanothermobacter thermautotrophicus |  |
| dithiothreitol | - |
Methanothermobacter thermautotrophicus | |
| additional information | maximal stimulation with 0.3 mM aquocobalamin and 0.15 mM Ti(III) citrate | Methanothermobacter thermautotrophicus | |
| reduced corrinoids | - |
Methanothermobacter thermautotrophicus | |
| Ti(III) citrate | - |
Methanothermobacter thermautotrophicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-bromoethanesulfonate | competitive with methyl coenzyme M | Methanothermobacter thermautotrophicus | |
| CoM-S-S-CoB | 50% inhibition at 0.6 mM | Methanothermobacter thermautotrophicus | |
| N-6-mercaptohexanoylthreonine phosphate | competitive with coenzyme B | Methanothermobacter thermautotrophicus | |
| N-7(methylthio)-heptanoylthreonine phosphate | competitive with coenzyme B | Methanothermobacter thermautotrophicus | |
| N-8-mercaptooctanoylthreonine phosphate | competitive with coenzyme B | Methanothermobacter thermautotrophicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
methyl coenzyme M | - |
Methanothermobacter thermautotrophicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | soluble | Methanothermobacter thermautotrophicus | 5737 | - |
| membrane | on the inner surface of the cytoplasmic membrane | Methanothermobacter thermautotrophicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni(2+) | - |
Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
operon contains two additional polypeptides of 15752 and 21210 Da with unknown functions | Methanothermobacter thermautotrophicus |
| 37000 | - |
2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 48000 | - |
2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 66000 | - |
2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 150000 | - |
PAGE | Methanothermobacter thermautotrophicus |
| 300000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus | final step in methane formation in all methanogenic archaea | methane + CoM-S-S-CoB | - |
? | |
| methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | final step in methane formation in all methanogenic archaea | methane + CoM-S-S-CoB | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| membrane filtration, on Mono Q, Superose-6 and Phenyl-Superose column | Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.075 | - |
up tp | Methanothermobacter thermautotrophicus |
| 0.5 | 2 | in presence of dithiothreitol and of reduced corrinoids or Ti(III) citrate | Methanothermobacter thermautotrophicus |
| 1 | 2 | in presence of 15 mM dithiothreitol and 0.3 mM aquocobalamin or 0.15 mM Ti(III) citrate | Methanothermobacter thermautotrophicus |
| 3 | 5 | cell extract | Methanothermobacter thermautotrophicus |
| 3 | 5 | drops to 1% after cell breakage | Methanothermobacter thermautotrophicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, under N2 atmosphere, 50 mM potassium phosphate, pH 6.7, 50% decrease of specific acitivity within 12 hours | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl coenzyme M + coenzyme B | final step in methane formation in all methanogenic archaea | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
| methyl coenzyme M + coenzyme B | final step in methane formation in all methanogenic archaea | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methane + CoM-S-S-CoB | - |
? | |
| methyl-coenzyme M + coenzyme B | specific for L-enantiomer of coenzyme B | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
| methyl-coenzyme M + coenzyme B | specific for L-enantiomer of coenzyme B | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methane + CoM-S-S-CoB | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | alpha2,beta2,gamma2 | Methanothermobacter thermautotrophicus |
| hexamer | 2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
assay at | Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
assay at | Methanothermobacter thermautotrophicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| F-430 | 1.6-1.8 mol F-430/mol enzyme | Methanothermobacter thermautotrophicus | |
| F-430 | nickel porphinoid coenzyme M | Methanothermobacter thermautotrophicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
N-6-mercaptohexanoylthreonine phosphate | competitive with coenzyme B | Methanothermobacter thermautotrophicus | |
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